Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA

Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA

<p>Abstract</p> <p>We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR in...

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Main Authors: Bruynseels Peggy, Verstrepen Walter A, Piqueur Marian AC, Mertens An H
Format: Article
Language:English
Published: BMC 2009-07-01
Series:Virology Journal
Online Access:http://www.virologyj.com/content/6/1/95
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spelling doaj-19ab4a1e86b148e2b97a1d4ffb6ce2932020-11-25T00:09:37ZengBMCVirology Journal1743-422X2009-07-01619510.1186/1743-422X-6-95Improvement of a real-time RT-PCR assay for the detection of enterovirus RNABruynseels PeggyVerstrepen Walter APiqueur Marian ACMertens An H<p>Abstract</p> <p>We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample.</p> http://www.virologyj.com/content/6/1/95
collection DOAJ
language English
format Article
sources DOAJ
author Bruynseels Peggy
Verstrepen Walter A
Piqueur Marian AC
Mertens An H
spellingShingle Bruynseels Peggy
Verstrepen Walter A
Piqueur Marian AC
Mertens An H
Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
Virology Journal
author_facet Bruynseels Peggy
Verstrepen Walter A
Piqueur Marian AC
Mertens An H
author_sort Bruynseels Peggy
title Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
title_short Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
title_full Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
title_fullStr Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
title_full_unstemmed Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
title_sort improvement of a real-time rt-pcr assay for the detection of enterovirus rna
publisher BMC
series Virology Journal
issn 1743-422X
publishDate 2009-07-01
description <p>Abstract</p> <p>We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample.</p>
url http://www.virologyj.com/content/6/1/95
work_keys_str_mv AT bruynseelspeggy improvementofarealtimertpcrassayforthedetectionofenterovirusrna
AT verstrepenwaltera improvementofarealtimertpcrassayforthedetectionofenterovirusrna
AT piqueurmarianac improvementofarealtimertpcrassayforthedetectionofenterovirusrna
AT mertensanh improvementofarealtimertpcrassayforthedetectionofenterovirusrna
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