Annexin A2 and zinc finger transcription factor Snail expression in glioma tissue and the regulating effect of corresponding siRNA on glioma cells

• Main Authors: Zheng-Hai Deng, Ying-Zhi Hou
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2016-12-01
• Series: Journal of Hainan Medical University
• Subjects: Glioma; Annexin A2; Snail; Proliferation; Epithelial-mesenchymal transition
• Online Access: http://www.hnykdxxb.com/PDF/201623/1.pdf

Objective: To study the Annexin A2 and zinc finger transcription factor Snail expression in glioma tissue and the regulating effect of corresponding siRNA on glioma cells. Methods: Glioma and peri-tumor tissue were collected to determine AnnexinA2 and Snail expression; glioma cell lines U373-MG were cultured and transfected with AnnexinA2, Snail and NC siRNA, and then the cell viability, number of migrating and invading cells as well as the expression levels of proliferation and epithelial-mesenchymal transition genes were detected. Results: AnnexinA2 and Snail mRNA levels in glioma tissues were significantly higher than those in peri-tumor tissues; cell viability as well as Ras, Raf, MEK and ERK mRNA levels of AnnexinA2-siRNA group was significantly lower than those of NC-siRNA group, and the migrating cell number and invading cell number as well as E-cadherin, N-cadherin, Vimentin and α-SMA mRNA levels were not significantly different from those of NC-siRNA group; migrating cell number and invading cell number as well as N-cadherin, Vimentin and α-SMA mRNA levels of Snail-siRNA group were significantly lower than those of NC-siRNA group, E-cadherin mRNA level was significantly higher than that of NC-siRNA group, and the cell viability as well as Ras, Raf, MEK and ERK mRNA levels were not significantly different from those of NC-siRNA group. Conclusions: AnnexinA2 and Snail expression levels significantly increase in glioma tissues, highly expressed AnnexinA2 can promote cell proliferation and highly expressed Snail can promote epithelial-mesenchymal transition.