Identification of two capa cDNA transcripts and detailed peptidomic characterization of their peptide products in Periplaneta americana

Identification of two capa cDNA transcripts and detailed peptidomic characterization of their peptide products in Periplaneta americana

The first CAPA peptides of insects were identified from Periplaneta americana using extracts of 8000 abdominal perisympathetic organs. The corresponding gene of cockroaches and other basal insects, however, remained undiscovered. Here we identified two capa cDNA transcripts of P. americana. Single c...

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Bibliographic Details
Main Authors: Susanne Neupert, Christian Derst, Sebastian Sturm, Reinhard Predel
Format: Article
Language:English
Published: Elsevier 2014-06-01
Series:EuPA Open Proteomics
Subjects:
Insect neuropeptide
Periviscerokinin
Pyrokinin
Single cell peptidomics
Alternative splicing
Tribolium
Online Access:http://www.sciencedirect.com/science/article/pii/S2212968514000099
Description
Summary:The first CAPA peptides of insects were identified from Periplaneta americana using extracts of 8000 abdominal perisympathetic organs. The corresponding gene of cockroaches and other basal insects, however, remained undiscovered. Here we identified two capa cDNA transcripts of P. americana. Single cell mass spectrometry of capa neurons in abdominal ganglia yielded a complete coverage of the precursor sequences of both transcripts; with intermediates of precursor processing more prominent in somata than in perisympathetic organs. The processing of the CAPA precursor in P. americana is compared with that in the beetle Tribolium castaneum which was re-evaluated in this study.
ISSN:2212-9685

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