Evaluation of the Diagnostic Potential of uPAR as a Biomarker in Renal Biopsies of Patients with FSGS
Minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS) are primary glomerulopathies leading to proteinuria, known as podocytopathies, which share syndromic and morphological similarities. Morphological similarity occurs in cases of FSGS in which the sclerotic lesion was not sampl...
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doaj-19575d6cfff1417fa687f90fe8141d472020-11-25T01:30:20ZengHindawi LimitedDisease Markers0278-02401875-86302019-01-01201910.1155/2019/10704951070495Evaluation of the Diagnostic Potential of uPAR as a Biomarker in Renal Biopsies of Patients with FSGSCrislaine Aparecida da Silva0Liliane Silvano Araújo1Maria Luíza Gonçalves dos Reis Monteiro2Lívia Helena de Morais Pereira3Marcos Vinícius da Silva4Lúcio Roberto Cançado Castellano5Rosana Rosa Miranda Corrêa6Marlene Antônia dos Reis7Juliana Reis Machado8Discipline of General Pathology, Institute of Biological and Natural Sciences of Federal University of Triângulo Mineiro, Praça Manoel Terra, 330, Nossa Senhora da Abadia, 38025-015 Uberaba, Minas Gerais, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences of Federal University of Triângulo Mineiro, Praça Manoel Terra, 330, Nossa Senhora da Abadia, 38025-015 Uberaba, Minas Gerais, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences of Federal University of Triângulo Mineiro, Praça Manoel Terra, 330, Nossa Senhora da Abadia, 38025-015 Uberaba, Minas Gerais, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences of Federal University of Triângulo Mineiro, Praça Manoel Terra, 330, Nossa Senhora da Abadia, 38025-015 Uberaba, Minas Gerais, BrazilDiscipline of Parasitology, Institute of Biological and Natural Sciences of Federal University of Triângulo Mineiro, Av. Getúlio Guaritá, No. 130, Nossa Senhora da Abadia, 38025-440 Uberaba, Minas Gerais, BrazilHuman Immunology Research and Education Group, Technical School of Health of Federal University of Paraíba, Cidade Universitária, 58059-900 João Pessoa, Paraíba, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences of Federal University of Triângulo Mineiro, Praça Manoel Terra, 330, Nossa Senhora da Abadia, 38025-015 Uberaba, Minas Gerais, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences of Federal University of Triângulo Mineiro, Praça Manoel Terra, 330, Nossa Senhora da Abadia, 38025-015 Uberaba, Minas Gerais, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences of Federal University of Triângulo Mineiro, Praça Manoel Terra, 330, Nossa Senhora da Abadia, 38025-015 Uberaba, Minas Gerais, BrazilMinimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS) are primary glomerulopathies leading to proteinuria, known as podocytopathies, which share syndromic and morphological similarities. Morphological similarity occurs in cases of FSGS in which the sclerotic lesion was not sampled in renal biopsy, due to the focal nature of the disease. Differentiating these entities is very important, especially in cases of suspected FSGS but with sclerotic lesion not sampled, as they are diseases that apparently have different pathogenic mechanisms and prognosis. The difference in uPAR expression in situ among these two entities may be related to a distinct molecular mechanism involved in pathogenesis. Thus, finding biomarkers involved in the pathogenesis and that can also help in differential diagnosis is very relevant. The aim of this work was to evaluate the potential of urokinase-type plasminogen activator receptor (uPAR) as a biomarker in renal biopsies of patients with podocytopathies (n=38). Immunohistochemistry showed that FSGS (n=22) had increased uPAR expression in podocytes compared with both the MCD group (n=16; p=0.0368) and control group (n=21; p=0.0076). ROC curve (p=0.008) showed that this biomarker has 80.95% of specificity in biopsies of patients with FSGS. Therefore, uPAR presented a high specificity in cases of podocytopathies associated with sclerosis and it can be considered a potential biomarker for FSGS.http://dx.doi.org/10.1155/2019/1070495 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Crislaine Aparecida da Silva Liliane Silvano Araújo Maria Luíza Gonçalves dos Reis Monteiro Lívia Helena de Morais Pereira Marcos Vinícius da Silva Lúcio Roberto Cançado Castellano Rosana Rosa Miranda Corrêa Marlene Antônia dos Reis Juliana Reis Machado |
spellingShingle |
Crislaine Aparecida da Silva Liliane Silvano Araújo Maria Luíza Gonçalves dos Reis Monteiro Lívia Helena de Morais Pereira Marcos Vinícius da Silva Lúcio Roberto Cançado Castellano Rosana Rosa Miranda Corrêa Marlene Antônia dos Reis Juliana Reis Machado Evaluation of the Diagnostic Potential of uPAR as a Biomarker in Renal Biopsies of Patients with FSGS Disease Markers |
author_facet |
Crislaine Aparecida da Silva Liliane Silvano Araújo Maria Luíza Gonçalves dos Reis Monteiro Lívia Helena de Morais Pereira Marcos Vinícius da Silva Lúcio Roberto Cançado Castellano Rosana Rosa Miranda Corrêa Marlene Antônia dos Reis Juliana Reis Machado |
author_sort |
Crislaine Aparecida da Silva |
title |
Evaluation of the Diagnostic Potential of uPAR as a Biomarker in Renal Biopsies of Patients with FSGS |
title_short |
Evaluation of the Diagnostic Potential of uPAR as a Biomarker in Renal Biopsies of Patients with FSGS |
title_full |
Evaluation of the Diagnostic Potential of uPAR as a Biomarker in Renal Biopsies of Patients with FSGS |
title_fullStr |
Evaluation of the Diagnostic Potential of uPAR as a Biomarker in Renal Biopsies of Patients with FSGS |
title_full_unstemmed |
Evaluation of the Diagnostic Potential of uPAR as a Biomarker in Renal Biopsies of Patients with FSGS |
title_sort |
evaluation of the diagnostic potential of upar as a biomarker in renal biopsies of patients with fsgs |
publisher |
Hindawi Limited |
series |
Disease Markers |
issn |
0278-0240 1875-8630 |
publishDate |
2019-01-01 |
description |
Minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS) are primary glomerulopathies leading to proteinuria, known as podocytopathies, which share syndromic and morphological similarities. Morphological similarity occurs in cases of FSGS in which the sclerotic lesion was not sampled in renal biopsy, due to the focal nature of the disease. Differentiating these entities is very important, especially in cases of suspected FSGS but with sclerotic lesion not sampled, as they are diseases that apparently have different pathogenic mechanisms and prognosis. The difference in uPAR expression in situ among these two entities may be related to a distinct molecular mechanism involved in pathogenesis. Thus, finding biomarkers involved in the pathogenesis and that can also help in differential diagnosis is very relevant. The aim of this work was to evaluate the potential of urokinase-type plasminogen activator receptor (uPAR) as a biomarker in renal biopsies of patients with podocytopathies (n=38). Immunohistochemistry showed that FSGS (n=22) had increased uPAR expression in podocytes compared with both the MCD group (n=16; p=0.0368) and control group (n=21; p=0.0076). ROC curve (p=0.008) showed that this biomarker has 80.95% of specificity in biopsies of patients with FSGS. Therefore, uPAR presented a high specificity in cases of podocytopathies associated with sclerosis and it can be considered a potential biomarker for FSGS. |
url |
http://dx.doi.org/10.1155/2019/1070495 |
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