Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC
The use of mobile phase additives enhances the separation and resolution of the bioactive compounds on the C18 column. Chlorogenic acid, caffeic acid, rutin, and scoparone from Herba Artemisiae Scopariae were investigated as the target compounds. Acetic acid, triethylamine, inorganic salts, and seve...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2010-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1422-0067/11/5/2229/ |
| id |
doaj-191c0cdecd8a4cc887a932934cf62952 |
|---|---|
| record_format |
Article |
| spelling |
doaj-191c0cdecd8a4cc887a932934cf629522020-11-25T01:58:31ZengMDPI AGInternational Journal of Molecular Sciences1422-00672010-05-011152229224010.3390/ijms11052229Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLCShengnan LiMinglei TianKyung Ho RowThe use of mobile phase additives enhances the separation and resolution of the bioactive compounds on the C18 column. Chlorogenic acid, caffeic acid, rutin, and scoparone from Herba Artemisiae Scopariae were investigated as the target compounds. Acetic acid, triethylamine, inorganic salts, and several ionic liquids were added as mobile phase additives into methanol/water (40:60, v/v). The result revealed that a mobile phase with 0.01 mol/L of ionic liquid [BMIM][BF4] enabled the optimum separation of the four target compounds. http://www.mdpi.com/1422-0067/11/5/2229/mobile phase additivebioactive compoundsionic liquidsHerba Artemisiae Scopariae |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Shengnan Li Minglei Tian Kyung Ho Row |
| spellingShingle |
Shengnan Li Minglei Tian Kyung Ho Row Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC International Journal of Molecular Sciences mobile phase additive bioactive compounds ionic liquids Herba Artemisiae Scopariae |
| author_facet |
Shengnan Li Minglei Tian Kyung Ho Row |
| author_sort |
Shengnan Li |
| title |
Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC |
| title_short |
Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC |
| title_full |
Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC |
| title_fullStr |
Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC |
| title_full_unstemmed |
Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC |
| title_sort |
effect of mobile phase additives on the resolution of four bioactive compounds by rp-hplc |
| publisher |
MDPI AG |
| series |
International Journal of Molecular Sciences |
| issn |
1422-0067 |
| publishDate |
2010-05-01 |
| description |
The use of mobile phase additives enhances the separation and resolution of the bioactive compounds on the C18 column. Chlorogenic acid, caffeic acid, rutin, and scoparone from Herba Artemisiae Scopariae were investigated as the target compounds. Acetic acid, triethylamine, inorganic salts, and several ionic liquids were added as mobile phase additives into methanol/water (40:60, v/v). The result revealed that a mobile phase with 0.01 mol/L of ionic liquid [BMIM][BF4] enabled the optimum separation of the four target compounds. |
| topic |
mobile phase additive bioactive compounds ionic liquids Herba Artemisiae Scopariae |
| url |
http://www.mdpi.com/1422-0067/11/5/2229/ |
| work_keys_str_mv |
AT shengnanli effectofmobilephaseadditivesontheresolutionoffourbioactivecompoundsbyrphplc AT mingleitian effectofmobilephaseadditivesontheresolutionoffourbioactivecompoundsbyrphplc AT kyunghorow effectofmobilephaseadditivesontheresolutionoffourbioactivecompoundsbyrphplc |
| _version_ |
1724969146741948416 |