The Correlations Between Concentrations of Myeloperoxidase, Serum Amyloid-A Protein and Scretory Phospolipase A-2 with Proinflammatory HDL in Healthy Male Person

• Main Authors: Marita Kaniawati, Andi Wijaya, Anwar Susanto
• Format: Article
• Language: English
• Published: Secretariat of The Indonesian Biomedical Journal 2009-04-01
• Series: Indonesian Biomedical Journal
• Online Access: http://inabj.org/index.php/ibj/article/view/83
• ISSN: 2085-3297; 2355-9179

BACKGROUND: Low-HDL cholesterol is a risk factor of CAD. Although levels of HDLC are within normal limit in some patients, they suffer CAD. These normal HDL-C levels might become pro-inflammatoric. This study is to measure the correlations between myeloperoxidase (MPO), serum amyloid-A (SAA) protein, and secretoryphospholipase-A2 (sPLA2) with inflammatory status of HDL-C. METHODS: This was a cross-sectional study recruited 49 subjects with high HDL-C (>40 mg/dL) and 31 subjects with low HDL-C (p<40 mg/dL). HDL-C was determined into antiinflammatory and proinflammatory based on levels of Apo A-1 and hs-CRP. Concentrations of MPO, SAA and s-PLA2 were measured by ELISA method. Levels of Apo A-1 was determined by immunoturbidimetric method. Multiple logistic regression analysis was done using inflammatory status of HDL-C as dependent variables and levels of MPO, SAA, sPLA2, ages, total cholesterol and triglycerides as independent variables. RESULTS: Patient’s age was 43.4+8.3 year, HDL-C was 43.1+9.5 mg/dL, Apo A-1 was 128.3+21.5 mg/dL, hs-CRP was 1.92+3.0 mg/dL. Concentrations of MPO, SAA and sPLA2 successively were 63.2+16.9 ng/mL, 7015.6+5021.1 ng/mL and 1340.2+406.3 pg/mL. Multiple logistic regression analysis showed that SAA is an independent predictor of pro-inflammatory status of HDL-C in high HDL-C group with prevalence ratio of 11.74 (95% CI: 2.51-54.84; p=0.002). In contrast, MPO and sPLA2 were not independent predictor with PR of 1.26 (95% CI: 0.30-5.23; p=0.75) and of 0.94 (95% CI: 0.23-3.91; p=0.93). CONCLUSIONS: SAA is an independent predictor of pro-inflammatory HDL-C even in subjects with high HDL-C. KEYWORDS: atherosclerosis, Apo A-1, serum amyloid A protein, secretory phospholipase A2, myeloperoxidase.