Application of detecting cerebrospinal fluid circulating tumor cells in the diagnosis of meningeal metastasis of non-small cell lung cancer

• Main Authors: Rong JIANG, Chun-hua MA, Zi-long ZHU, Jin-duo LI, Bin WANG, Li-wei SUN, Yuan LÜ
• Format: Article
• Language: English
• Published: Tianjin Huanhu Hospital 2014-08-01
• Series: Chinese Journal of Contemporary Neurology and Neurosurgery
• Subjects: Neoplasm circulating cells; Cerebrospinal fluid; Carcinoma, non-small-cell lung; Neoplasm metastasis; Meningeal neoplasms; In situ hybridization, fluorescence
• Online Access: http://www.cjcnn.org/index.php/cjcnn/article/view/1018

<p><strong>Objective</strong> To observe a new technology for the detection and enumeration of cerebrospinal fluid (CSF) circulating tumor cells (CTCs) in the diagnosis of non-small cell lung cancer (NSCLC) with meningeal metastasis (MM).  <strong>Methods</strong> Five cases of NSCLC with MM that were diagnosed by CSF cytology were selected, and 20 ml CSF samples were obtained by lumbar puncture for every patient. The tumor marker immunostaining-fluorescence in situ hybridization (TM-iFISH) technology was adapted to detect enrichment and enumeration of circulating tumor cells in 7.50 ml CSF samples; CSF cytology was checked in 10 ml CSF samples; CSF tumor markers were detected in 2.50 ml CSF samples. All of 5 cases were examined by MRI enhancement scan.  <strong>Results</strong> TM-iFISH detection found circulating tumor cells numbers ranging 18-1823/7.50 ml. Only 2 cases of patients with CSF cytology examination showed the tumor cells. The results of CSF tumor markers in all samples were higher than normal serum tumor markers detection results. The enhanced MRI scan of 5 cases revealed typical signs of MM.  <strong>Conclusions</strong> The TM-iFISH test showed certain advantages in the detection of malignant tumor cells in CSF. This technology may be a new method of detection and enumeration of tumor cells in CSF, but more studies are needed to prove its sensitivity and specificity.</p><p> </p><p>doi: 10.3969/j.issn.1672-6731.2014.08.011</p>