| Summary: | Chromium is one of main pollution in sea water, and the establishment of detecting kit is the research hot topic. In this study, immunized mice were obtained by immunogen Cr3+-EDTA-BSA, and hybridoma strains that secrete Cr3+-EDTA monoclonal antibody were selected. Cr3+-EDTA mAb were prepared with in vivo method. Then, Cr-kit for measurement of chromium was developed, and its application was executed in sea water and seafood. Two hybridoma strains of A321 and A424 were screened. The Cr-kit standard curve of the linear range was 1.0—512 μg/L, and limit of detection was 1.0 μg/L. The average recovery rates Cr spiked in samples of sea water, clam and shrimp were 102.62%, 94.69% and 96.29%, which were accordance with the results of ICP-AES. Totally, Cr-kit was successfully developed and behaved broad application potential in sea water and seafood.
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