Detection of virulence genes in Uropathogenic E. coli (UPEC) strains by Multiplex-PCR method

• Main Authors: Javad Mohammadi, Kumarss Amini
• Format: Article
• Language: fas
• Published: Fasa University of Medical Sciences 2017-06-01
• Series: Journal of Fasa University of Medical Sciences
• Subjects: UPEC; iha; iroN; ompT; multiplex-PCR
• Online Access: http://journal.fums.ac.ir/browse.php?a_code=A-10-839-1&slc_lang=en&sid=1

Background & Objectives: Urinary tract infection caused by E. coli is one of the most common illnesses in all age groups worldwide. Presence of virulence genes is a key factor in bacterial pathogens in uroepithelial cells. The present study was performed to detect iha, iroN, ompT genes in the Uropathogenic E.coli isolates from clinical samples using multiplex-PCR method in Kerman. Materials & Methods: In this descriptive cross-sectional study, 200 samples of patients with urinary tract infections in Kerman hospitals were collected. After biochemical and microbiological tests, all strains were tested with regard to the presence of iha, iroN, and ompT genes using multiplex-PCR method. Results: The results of Multiplex-PCR showed that all specimens had one, two, or three virulence genes simultaneously. The highest and lowest frequency distribution of genes was related to iha (56.7%) and iroN (20%) respectively. Conclusion: According to the prevalence of urinary tract infection in the community and distribution of resistance and virulence factors, the fast and accurate detection of the strains and virulence genes is necessary