Generation of KCL028 research grade human embryonic stem cell line carrying a mutation in the HTT gene

Generation of KCL028 research grade human embryonic stem cell line carrying a mutation in the HTT gene

The KCL028 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (43 trinucleotide repeats; 21 for the normal allele). The ICM was isolated using laser microsurgery and plate...

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Main Authors: Laureen Jacquet, Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Carl Hobbs, Emma Stephenson, Dusko Ilic
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506116000167
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spelling doaj-18b2192a7e9b4dd0bd6040b0e4acb4272020-11-24T21:54:34ZengElsevierStem Cell Research1873-50611876-77532016-03-0116227828110.1016/j.scr.2016.01.006Generation of KCL028 research grade human embryonic stem cell line carrying a mutation in the HTT geneLaureen Jacquet0Heema Hewitson1Victoria Wood2Neli Kadeva3Glenda Cornwell4Stefano Codognotto5Carl Hobbs6Emma Stephenson7Dusko Ilic8Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guy's Hospital, London, United KingdomStem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guy's Hospital, London, United KingdomStem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guy's Hospital, London, United KingdomStem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guy's Hospital, London, United KingdomStem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guy's Hospital, London, United KingdomStem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guy's Hospital, London, United KingdomHistology Laboratory, Wolfson Centre for Age-Related Diseases, Faculty of Life Sciences and Medicine, King's College London, London, United KingdomStem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guy's Hospital, London, United KingdomStem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guy's Hospital, London, United KingdomThe KCL028 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (43 trinucleotide repeats; 21 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.http://www.sciencedirect.com/science/article/pii/S1873506116000167
collection DOAJ
language English
format Article
sources DOAJ
author Laureen Jacquet
Heema Hewitson
Victoria Wood
Neli Kadeva
Glenda Cornwell
Stefano Codognotto
Carl Hobbs
Emma Stephenson
Dusko Ilic
spellingShingle Laureen Jacquet
Heema Hewitson
Victoria Wood
Neli Kadeva
Glenda Cornwell
Stefano Codognotto
Carl Hobbs
Emma Stephenson
Dusko Ilic
Generation of KCL028 research grade human embryonic stem cell line carrying a mutation in the HTT gene
Stem Cell Research
author_facet Laureen Jacquet
Heema Hewitson
Victoria Wood
Neli Kadeva
Glenda Cornwell
Stefano Codognotto
Carl Hobbs
Emma Stephenson
Dusko Ilic
author_sort Laureen Jacquet
title Generation of KCL028 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_short Generation of KCL028 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_full Generation of KCL028 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_fullStr Generation of KCL028 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_full_unstemmed Generation of KCL028 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_sort generation of kcl028 research grade human embryonic stem cell line carrying a mutation in the htt gene
publisher Elsevier
series Stem Cell Research
issn 1873-5061
1876-7753
publishDate 2016-03-01
description The KCL028 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (43 trinucleotide repeats; 21 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.
url http://www.sciencedirect.com/science/article/pii/S1873506116000167
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