A Molecular Imprinted Polymer as a Flow-Through Optical Sensor for Oxazepam

A flow-through optosensing system for oxazepam recognition with fluorescence detection was performed by means of a molecular imprinted polymer based on its acid hydrolysis product, 2-amino-5-chlorobenzophenone. The synthesis was conducted via a noncovalent imprinting methodology, using methacrylic a...

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Main Authors:	Roberta G. Machicote, Marcela A. Castillo, Maria E. Pacheco, Liliana Bruzzone
Format:	Article
Language:	English
Published:	Hindawi Limited 2018-01-01
Series:	Journal of Analytical Methods in Chemistry
Online Access:	http://dx.doi.org/10.1155/2018/6302609

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spelling	doaj-18805691035440e786325c2cecc633a92020-11-25T00:53:46ZengHindawi LimitedJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/63026096302609A Molecular Imprinted Polymer as a Flow-Through Optical Sensor for OxazepamRoberta G. Machicote0Marcela A. Castillo1Maria E. Pacheco2Liliana Bruzzone3División Química Analítica, Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47, Esq. 115, 1900 La Plata, ArgentinaDivisión Química Analítica, Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47, Esq. 115, 1900 La Plata, ArgentinaLaboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47, Esq. 115, 1900 La Plata, ArgentinaLaboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47, Esq. 115, 1900 La Plata, ArgentinaA flow-through optosensing system for oxazepam recognition with fluorescence detection was performed by means of a molecular imprinted polymer based on its acid hydrolysis product, 2-amino-5-chlorobenzophenone. The synthesis was conducted via a noncovalent imprinting methodology, using methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a cross-linking agent. Hydrolysis (types and concentration of acids), polymer retention capacity, binding properties, and elution (selectivity and reversibility) conditions were optimized. The selected molecular imprinted polymer had a molar ratio composition of 1 : 6 : 45 (template : functional monomer : cross-linker). The proposed method was applied to the determination of oxazepam in a pharmaceutical formulation. External standard calibration, standard additions calibration, and Youden’s calibration were carried out in order to evaluate constant and proportional errors due to the matrix. The developed metabolite-based recognition system for benzodiazepines is an innovative procedure that could be followed in routine and quality control assays.http://dx.doi.org/10.1155/2018/6302609
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Roberta G. Machicote Marcela A. Castillo Maria E. Pacheco Liliana Bruzzone
spellingShingle	Roberta G. Machicote Marcela A. Castillo Maria E. Pacheco Liliana Bruzzone A Molecular Imprinted Polymer as a Flow-Through Optical Sensor for Oxazepam Journal of Analytical Methods in Chemistry
author_facet	Roberta G. Machicote Marcela A. Castillo Maria E. Pacheco Liliana Bruzzone
author_sort	Roberta G. Machicote
title	A Molecular Imprinted Polymer as a Flow-Through Optical Sensor for Oxazepam
title_short	A Molecular Imprinted Polymer as a Flow-Through Optical Sensor for Oxazepam
title_full	A Molecular Imprinted Polymer as a Flow-Through Optical Sensor for Oxazepam
title_fullStr	A Molecular Imprinted Polymer as a Flow-Through Optical Sensor for Oxazepam
title_full_unstemmed	A Molecular Imprinted Polymer as a Flow-Through Optical Sensor for Oxazepam
title_sort	molecular imprinted polymer as a flow-through optical sensor for oxazepam
publisher	Hindawi Limited
series	Journal of Analytical Methods in Chemistry
issn	2090-8865 2090-8873
publishDate	2018-01-01
description	A flow-through optosensing system for oxazepam recognition with fluorescence detection was performed by means of a molecular imprinted polymer based on its acid hydrolysis product, 2-amino-5-chlorobenzophenone. The synthesis was conducted via a noncovalent imprinting methodology, using methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a cross-linking agent. Hydrolysis (types and concentration of acids), polymer retention capacity, binding properties, and elution (selectivity and reversibility) conditions were optimized. The selected molecular imprinted polymer had a molar ratio composition of 1 : 6 : 45 (template : functional monomer : cross-linker). The proposed method was applied to the determination of oxazepam in a pharmaceutical formulation. External standard calibration, standard additions calibration, and Youden’s calibration were carried out in order to evaluate constant and proportional errors due to the matrix. The developed metabolite-based recognition system for benzodiazepines is an innovative procedure that could be followed in routine and quality control assays.
url	http://dx.doi.org/10.1155/2018/6302609
work_keys_str_mv	AT robertagmachicote amolecularimprintedpolymerasaflowthroughopticalsensorforoxazepam AT marcelaacastillo amolecularimprintedpolymerasaflowthroughopticalsensorforoxazepam AT mariaepacheco amolecularimprintedpolymerasaflowthroughopticalsensorforoxazepam AT lilianabruzzone amolecularimprintedpolymerasaflowthroughopticalsensorforoxazepam AT robertagmachicote molecularimprintedpolymerasaflowthroughopticalsensorforoxazepam AT marcelaacastillo molecularimprintedpolymerasaflowthroughopticalsensorforoxazepam AT mariaepacheco molecularimprintedpolymerasaflowthroughopticalsensorforoxazepam AT lilianabruzzone molecularimprintedpolymerasaflowthroughopticalsensorforoxazepam
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A Molecular Imprinted Polymer as a Flow-Through Optical Sensor for Oxazepam

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