A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

Dynamic labeling and tracking of organelle–organelle contacts is essential to understand the formation and function of these interactions. Here the authors present a small molecule probe, Coupa, that labels mitochondria and lysosomes with blue and red fluorescence, respectively.

Bibliographic Details
Main Authors:	Qixin Chen, Hongbao Fang, Xintian Shao, Zhiqi Tian, Shanshan Geng, Yuming Zhang, Huaxun Fan, Pan Xiang, Jie Zhang, Xiaohe Tian, Kai Zhang, Weijiang He, Zijian Guo, Jiajie Diao
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-12-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-20067-6

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spelling	doaj-1874dd6bf6214e2ba26b5f13d4206d542021-01-31T13:34:38ZengNature Publishing GroupNature Communications2041-17232020-12-0111111010.1038/s41467-020-20067-6A dual-labeling probe to track functional mitochondria–lysosome interactions in live cellsQixin Chen0Hongbao Fang1Xintian Shao2Zhiqi Tian3Shanshan Geng4Yuming Zhang5Huaxun Fan6Pan Xiang7Jie Zhang8Xiaohe Tian9Kai Zhang10Weijiang He11Zijian Guo12Jiajie Diao13State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing UniversityState Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing UniversityDepartment of Cancer Biology, University of Cincinnati College of MedicineDepartment of Cancer Biology, University of Cincinnati College of MedicineState Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing UniversityState Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing UniversityDepartment of Biochemistry, University of Illinois at Urbana-ChampaignAnhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Anhui UniversityAdvanced Medical Research Institute/Translational Medicine Core Facility of Advanced Medical Research Institute, Shandong UniversityAnhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Anhui UniversityDepartment of Biochemistry, University of Illinois at Urbana-ChampaignState Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing UniversityState Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing UniversityDepartment of Cancer Biology, University of Cincinnati College of MedicineDynamic labeling and tracking of organelle–organelle contacts is essential to understand the formation and function of these interactions. Here the authors present a small molecule probe, Coupa, that labels mitochondria and lysosomes with blue and red fluorescence, respectively.https://doi.org/10.1038/s41467-020-20067-6
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Qixin Chen Hongbao Fang Xintian Shao Zhiqi Tian Shanshan Geng Yuming Zhang Huaxun Fan Pan Xiang Jie Zhang Xiaohe Tian Kai Zhang Weijiang He Zijian Guo Jiajie Diao
spellingShingle	Qixin Chen Hongbao Fang Xintian Shao Zhiqi Tian Shanshan Geng Yuming Zhang Huaxun Fan Pan Xiang Jie Zhang Xiaohe Tian Kai Zhang Weijiang He Zijian Guo Jiajie Diao A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells Nature Communications
author_facet	Qixin Chen Hongbao Fang Xintian Shao Zhiqi Tian Shanshan Geng Yuming Zhang Huaxun Fan Pan Xiang Jie Zhang Xiaohe Tian Kai Zhang Weijiang He Zijian Guo Jiajie Diao
author_sort	Qixin Chen
title	A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells
title_short	A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells
title_full	A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells
title_fullStr	A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells
title_full_unstemmed	A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells
title_sort	dual-labeling probe to track functional mitochondria–lysosome interactions in live cells
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-12-01
description	Dynamic labeling and tracking of organelle–organelle contacts is essential to understand the formation and function of these interactions. Here the authors present a small molecule probe, Coupa, that labels mitochondria and lysosomes with blue and red fluorescence, respectively.
url	https://doi.org/10.1038/s41467-020-20067-6
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A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

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