A novel biomarker TERTmRNA is applicable for early detection of hepatoma
<p>Abstract</p> <p>Backgrounds</p> <p>We previously reported a highly sensitive method for serum human telomerase reverse transcriptase (hTERT) mRNA for hepatocellular carcinoma (HCC). α-fetoprotein (AFP) and des-γ-carboxy prothrombin (DCP) are good markers for HCC. In...
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doaj-1863e75e19024bafa300f68a236fe6aa2020-11-25T01:42:42ZengBMCBMC Gastroenterology1471-230X2010-05-011014610.1186/1471-230X-10-46A novel biomarker TERTmRNA is applicable for early detection of hepatomaHirooka YasuakiSakaguchi SeigoKudo MasatoshiHorie YutakaNoma EijiroMaruyama ShigeoKishimoto YukihiroOyama KenjiKanbe TakamasaShomori KoheiYorozu KenshoNagashima MikiKohno MichimoriOsaki YukioMiura NorimasaIto HisaoKawasaki HironakaHasegawa JunichiShiota Goshi<p>Abstract</p> <p>Backgrounds</p> <p>We previously reported a highly sensitive method for serum human telomerase reverse transcriptase (hTERT) mRNA for hepatocellular carcinoma (HCC). α-fetoprotein (AFP) and des-γ-carboxy prothrombin (DCP) are good markers for HCC. In this study, we verified the significance of hTERTmRNA in a large scale multi-centered trial, collating quantified values with clinical course.</p> <p>Methods</p> <p>In 638 subjects including 303 patients with HCC, 89 with chronic hepatitis (CH), 45 with liver cirrhosis (LC) and 201 healthy individuals, we quantified serum hTERTmRNA using the real-time RT-PCR. We examined its sensitivity and specificity in HCC diagnosis, clinical significance, ROC curve analysis in comparison with other tumor markers, and its correlations with the clinical parameters using Pearson relative test and multivariate analyses. Furthermore, we performed a prospective and comparative study to observe the change of biomarkers, including hTERTmRNA in HCC patients receiving anti-cancer therapies.</p> <p>Results</p> <p>hTERTmRNA was demonstrated to be independently correlated with clinical parameters; tumor size and tumor differentiation (P < 0.001, each). The sensitivity/specificity of hTERTmRNA in HCC diagnosis showed 90.2%/85.4% for hTERT. hTERTmRNA proved to be superior to AFP, AFP-L3, and DCP in the diagnosis and underwent an indisputable change in response to therapy. The detection rate of small HCC by hTERTmRNA was superior to the other markers.</p> <p>Conclusions</p> <p>hTERTmRNA is superior to conventional tumor markers in the diagnosis and recurrence of HCC at an early stage.</p> http://www.biomedcentral.com/1471-230X/10/46 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hirooka Yasuaki Sakaguchi Seigo Kudo Masatoshi Horie Yutaka Noma Eijiro Maruyama Shigeo Kishimoto Yukihiro Oyama Kenji Kanbe Takamasa Shomori Kohei Yorozu Kensho Nagashima Miki Kohno Michimori Osaki Yukio Miura Norimasa Ito Hisao Kawasaki Hironaka Hasegawa Junichi Shiota Goshi |
spellingShingle |
Hirooka Yasuaki Sakaguchi Seigo Kudo Masatoshi Horie Yutaka Noma Eijiro Maruyama Shigeo Kishimoto Yukihiro Oyama Kenji Kanbe Takamasa Shomori Kohei Yorozu Kensho Nagashima Miki Kohno Michimori Osaki Yukio Miura Norimasa Ito Hisao Kawasaki Hironaka Hasegawa Junichi Shiota Goshi A novel biomarker TERTmRNA is applicable for early detection of hepatoma BMC Gastroenterology |
author_facet |
Hirooka Yasuaki Sakaguchi Seigo Kudo Masatoshi Horie Yutaka Noma Eijiro Maruyama Shigeo Kishimoto Yukihiro Oyama Kenji Kanbe Takamasa Shomori Kohei Yorozu Kensho Nagashima Miki Kohno Michimori Osaki Yukio Miura Norimasa Ito Hisao Kawasaki Hironaka Hasegawa Junichi Shiota Goshi |
author_sort |
Hirooka Yasuaki |
title |
A novel biomarker TERTmRNA is applicable for early detection of hepatoma |
title_short |
A novel biomarker TERTmRNA is applicable for early detection of hepatoma |
title_full |
A novel biomarker TERTmRNA is applicable for early detection of hepatoma |
title_fullStr |
A novel biomarker TERTmRNA is applicable for early detection of hepatoma |
title_full_unstemmed |
A novel biomarker TERTmRNA is applicable for early detection of hepatoma |
title_sort |
novel biomarker tertmrna is applicable for early detection of hepatoma |
publisher |
BMC |
series |
BMC Gastroenterology |
issn |
1471-230X |
publishDate |
2010-05-01 |
description |
<p>Abstract</p> <p>Backgrounds</p> <p>We previously reported a highly sensitive method for serum human telomerase reverse transcriptase (hTERT) mRNA for hepatocellular carcinoma (HCC). α-fetoprotein (AFP) and des-γ-carboxy prothrombin (DCP) are good markers for HCC. In this study, we verified the significance of hTERTmRNA in a large scale multi-centered trial, collating quantified values with clinical course.</p> <p>Methods</p> <p>In 638 subjects including 303 patients with HCC, 89 with chronic hepatitis (CH), 45 with liver cirrhosis (LC) and 201 healthy individuals, we quantified serum hTERTmRNA using the real-time RT-PCR. We examined its sensitivity and specificity in HCC diagnosis, clinical significance, ROC curve analysis in comparison with other tumor markers, and its correlations with the clinical parameters using Pearson relative test and multivariate analyses. Furthermore, we performed a prospective and comparative study to observe the change of biomarkers, including hTERTmRNA in HCC patients receiving anti-cancer therapies.</p> <p>Results</p> <p>hTERTmRNA was demonstrated to be independently correlated with clinical parameters; tumor size and tumor differentiation (P < 0.001, each). The sensitivity/specificity of hTERTmRNA in HCC diagnosis showed 90.2%/85.4% for hTERT. hTERTmRNA proved to be superior to AFP, AFP-L3, and DCP in the diagnosis and underwent an indisputable change in response to therapy. The detection rate of small HCC by hTERTmRNA was superior to the other markers.</p> <p>Conclusions</p> <p>hTERTmRNA is superior to conventional tumor markers in the diagnosis and recurrence of HCC at an early stage.</p> |
url |
http://www.biomedcentral.com/1471-230X/10/46 |
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