Combining the Physical Adsorption Approach and the Covalent Attachment Method to Prepare a Bifunctional Bioreactor

• Main Authors: Zhi Wang, Zhengqiang Li, Ming Lu, Zhuofu Wu, Mengxing Dong
• Format: Article
• Language: English
• Published: MDPI AG 2012-09-01
• Series: International Journal of Molecular Sciences
• Subjects: adsorption; amino-functionalized mesoporous silica; covalent attachment; myoglobin; lysozyme; peroxidase activity; antibacterial activity
• Online Access: http://www.mdpi.com/1422-0067/13/9/11443

Aminopropyl-functionalized SBA-15 mesoporous silica was used as a support to adsorb myoglobin. Then, in order to avoid the leakage of adsorbed myoglobin, lysozyme was covalently tethered to the internal and external surface of the mesoporous silica with glutaraldehyde as the coupling agent. The property of amino-functionalized mesoporous silica was characterized by N2 adsorption-desorption and thermogravimetric (TG) analysis. The feature of the silica-based matrix before and after myoglobin adsorption was identified by fourier transform infrared (FTIR) and UV/VIS measurement. With o-dianisidine and H2O2 as the substrate, the peroxidase activity of adsorbed myoglobin was determined. With Micrococus lysodeilicus as the substrate, the antibacterial activity of covalently tethered lysozyme was measured. Results demonstrated that the final product not only presented peroxidase activity of the myoglobin but yielded antibacterial activity of the lysozyme.