The significance of M1/M2 macrophage-like monocytes in children with systemic lupus erythematosus

• Main Authors: Xiao-Ling Niu, Dan Feng, Sheng Hao, Xin-Yu Kuang, Ying Wu, Guang-Hua Zhu, Wen-Yan Huang
• Format: Article
• Language: English
• Published: SAGE Publishing 2019-01-01
• Series: European Journal of Inflammation
• Online Access: https://doi.org/10.1177/2058739218824463

Monocytes/macrophages are important in the development of systemic lupus erythematosus. To research M1 and M2 macrophage-like monocytes in the peripheral blood of children with systemic lupus erythematosus and explore the clinical significance, M1 and M2 macrophage-like monocytes, tumor necrosis factor-α, interleukin-1, interleukin-6, interleukin-10, and interleukin-18 are tested in the peripheral blood of children with systemic lupus erythematosus by flow cytometry. A correlation analysis is made between M1 and M2 macrophage-like monocytes and erythrocyte sedimentation rate and C-reactive protein. As we found, the absolute number and percentage of M1 macrophage-like monocytes (CD163 – CD14 + ) in macrophage-like monocytes (CD14 + ) in peripheral blood of the severe systemic lupus erythematosus group were higher than those of the control group and the mild–moderate systemic lupus erythematosus group ( F  = 28.4, 21.7, 122, 81.7; P  < 0.05). But there was no obvious difference between these three groups in terms of the absolute number of M2 macrophage-like monocytes (CD163 + CD14 + ). The absolute number and percentage of M1 macrophage-like monocytes in macrophage-like monocytes had positive correlation with C-reactive protein and erythrocyte sedimentation rate ( r   = 0.46, 0.44, 0.367, 0.47; P  < 0.05); whereas, the absolute number and percentage of M2 macrophage-like monocytes in macrophage-like monocytes had negative correlation with CRP and erythrocyte sedimentation rate ( r  = –0.47, –0.45, –0.47, –0.32; P  < 0.05). Thus, M1 macrophage-like monocytes have effective impact on inflammation in children with systemic lupus erythematosus. M2 macrophage-like monocytes, to a large extent, have the opposite functions compared to M1 macrophage-like monocytes. In children with systemic lupus erythematosus, macrophages play important role in the development of systemic lupus erythematosus and M1 macrophage-like monocytes have functions in active systemic lupus erythematosus and they can induce the inflammation and have correlation with severity of systemic lupus erythematosus.