Analysis of Alicyclobacillus acidoterrestris spores from different sporulation media subjected to wet-heat

• Main Authors: Celenk Molva, Ayse Handan Baysal
• Format: Article
• Language: English
• Published: MDPI AG 2015-12-01
• Series: Microbiology Research
• Subjects: Wet-heat; sporulation media; scanning electron microscopy; leakage
• Online Access: http://www.pagepress.org/journals/index.php/mr/article/view/6219

The presence of <em>Alicyclobacillus acidoterrestris</em> endospores in fruit juices is a significant problem for the juice industry since they are able to survive pasteurization subsequently leading to the spoilage. To evaluate the mechanism of wet-heat, structural damage and the leakages of intracellular materials of A. acidoterrestris DSM 3922 spores from different sporulation media was studied at 90°C (15-45 min). For sporulation, <em>Bacillus</em> <em>acidoterrestris</em> agar, <em>Bacillus acidocaldarius</em> agar, potato dextrose agar and malt extract agar were used. Based on the scanning electron microscopy, loss of internal volume and structural integrity were observed following heating which were further confirmed by the leakages of intracellular components. The obtained results suggest that the inactivation of <em>A. acidoterrestris</em> DSM 3922 spores by wet-heat is associated with damage to the coat and inner membrane depending on the sporulation medium composition and heating time.