Reference gene selection for qPCR is dependent on cell type rather than treatment in colonic and vaginal human epithelial cell lines.
The ability of commensal bacteria to influence gene expression in host cells under the influence of pathogenic bacteria has previously been demonstrated, however the extent of this interaction is important for understanding how bacteria can be used as probiotics. Real-time quantitative polymerase ch...
Main Authors: | Annette V Jacobsen, Bisrat T Yemaneab, Jana Jass, Nikolai Scherbak |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4272277?pdf=render |
Similar Items
-
Comparison of qPCR and culture methods for group B Streptococcus colonization detection in pregnant women: evaluation of a new qPCR assay
by: J. A. Carrillo-Ávila, et al.
Published: (2018-07-01) -
Single Cell RT-qPCR on 3D Cell Spheroids
Published: (2016) -
qPCR data analysis: Better results through iconoclasm
by: Joel Tellinghuisen, et al.
Published: (2019-03-01) -
Possibilities of qPCR control of mycoplasma contamination of cell cultures
by: N. D. Yolshin, et al.
Published: (2018-02-01) -
qPCR primer design revisited
by: Stephen Bustin, et al.
Published: (2017-12-01)