Production and Optimization of Oleic Acid Ethyl Ester Synthesis Using Lipase From Rice Bran (Oryza sativa L.) and Germinated Jatropha Seeds (Jatropha curcas L.) by Response Surface Methodology

• Main Authors: Indro Prastowo, Chusnul Hidayat, Pramudji Hastuti
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada, Yogyakarta 2015-11-01
• Series: Indonesian Journal of Biotechnology
• Online Access: http://journal.ugm.ac.id/ijbiotech/article/view/7848

<!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:UseFELayout /> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--> <p class="MsoNormal" style="mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 9.5pt; font-family: BookAntiqua; mso-bidi-font-family: BookAntiqua;">Recently, the fatty acid ethyl ester has been synthesized in place of fatty acid methyl ester since ethanol</span></p> <p class="MsoNormal" style="mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 9.5pt; font-family: BookAntiqua; mso-bidi-font-family: BookAntiqua;">has been more renewable. In this research, oleic acid ethyl ester (OAEE) was synthesized using germinated</span></p> <p class="MsoNormal" style="mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 9.5pt; font-family: BookAntiqua; mso-bidi-font-family: BookAntiqua;">jatropha seeds (</span><em><span style="font-size: 9.5pt; font-family: BookAntiqua-Italic; mso-bidi-font-family: BookAntiqua-Italic;">Jatropha curcas.</span></em><span style="font-size: 9.5pt; font-family: BookAntiqua; mso-bidi-font-family: BookAntiqua;">L) and rice bran </span><strong><span style="font-size: 9.5pt; font-family: BookAntiqua-Bold; mso-bidi-font-family: BookAntiqua-Bold;">(</span></strong><em><span style="font-size: 9.5pt; font-family: BookAntiqua-Italic; mso-bidi-font-family: BookAntiqua-Italic;">Oryza sativa</span></em><span style="font-size: 9.5pt; font-family: BookAntiqua; mso-bidi-font-family: BookAntiqua;">) as source of lipase. The objective of the research was to optimize the synthesis conditions using </span><em><span style="font-size: 9.5pt; font-family: BookAntiqua-Italic; mso-bidi-font-family: BookAntiqua-Italic;">Response Surface Methodology</span></em><span style="font-size: 9.5pt; font-family: BookAntiqua; mso-bidi-font-family: BookAntiqua;">. Factors, such as crude enzyme concentration, molar ratio of oleic acid to ethanol, and the reaction time, were evaluated. The results show that lipase from germinated jatropha seeds had the hydrolitic and esterifi cation activity about 6.73 U/g and 298.07 U/g, respectively. Lipase from rice bran had the hydrolitic and esterifi cation activity about 10.57 U/g and 324.03 U/g, respectively. The optimum conditions of esterifi cation reaction using germinated jatropha</span></p> <p class="MsoNormal" style="mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 9.5pt; font-family: BookAntiqua; mso-bidi-font-family: BookAntiqua;">seed lipase as biocatalyst were crude enzyme concentration of 0.31 g/ml, molar ratio of oleic acid to ethanol of</span></p> <p class="MsoNormal" style="mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 9.5pt; font-family: BookAntiqua; mso-bidi-font-family: BookAntiqua;">1 : 1.81, and reaction time of 50.9 min. The optimum conditions of esterifi cation reaction using rice bran lipase</span></p> <p class="MsoNormal" style="mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 9.5pt; font-family: BookAntiqua; mso-bidi-font-family: BookAntiqua;">were crude enzyme concentration of 0.29 g/ml, molar ratio of oleic acid to ethanol of 1 : 2.05, and reaction</span></p> <p class="MsoNormal" style="mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 9.5pt; font-family: BookAntiqua; mso-bidi-font-family: BookAntiqua;">time of 58.61 min. The obtained amounts of OAEE were 810.77 &mu;mole and 626.92 &mu;mole for lipases from rice</span></p> <p class="MsoNormal" style="mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 9.5pt; font-family: BookAntiqua; mso-bidi-font-family: BookAntiqua;">bran and germinated jatropha seed, respectively.</span></p> <!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} </style> <![endif]-->