The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents

This paper presents a systematic investigation into monomer development during mixed culture Polyhydroxyalkanoates (PHA) accumulation involving concurrent active biomass growth and polymer storage. A series of mixed culture PHA accumulation experiments, using several different substrate-feeding stra...

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Main Authors: Liliana Montano-Herrera, Bronwyn Laycock, Alan Werker, Steven Pratt
Format: Article
Language:English
Published: MDPI AG 2017-03-01
Series:Bioengineering
Subjects:
PHA
Online Access:http://www.mdpi.com/2306-5354/4/1/20
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spelling doaj-17fe3c29b4244271a0fdc52e07d608182020-11-24T22:37:57ZengMDPI AGBioengineering2306-53542017-03-01412010.3390/bioengineering4010020bioengineering4010020The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing EquivalentsLiliana Montano-Herrera0Bronwyn Laycock1Alan Werker2Steven Pratt3School of Chemical Engineering, University of Queensland, St Lucia QLD 4072, AustraliaSchool of Chemical Engineering, University of Queensland, St Lucia QLD 4072, AustraliaVeolia Water Technologies AB—AnoxKaldnes, Klosterängsvägen 11A SE-226 47 Lund, SwedenSchool of Chemical Engineering, University of Queensland, St Lucia QLD 4072, AustraliaThis paper presents a systematic investigation into monomer development during mixed culture Polyhydroxyalkanoates (PHA) accumulation involving concurrent active biomass growth and polymer storage. A series of mixed culture PHA accumulation experiments, using several different substrate-feeding strategies, was carried out. The feedstock comprised volatile fatty acids, which were applied as single carbon sources, as mixtures, or in series, using a fed-batch feed-on-demand controlled bioprocess. A dynamic trend in active biomass growth as well as polymer composition was observed. The observations were consistent over replicate accumulations. Metabolic flux analysis (MFA) was used to investigate metabolic activity through time. It was concluded that carbon flux, and consequently copolymer composition, could be linked with how reducing equivalents are generated.http://www.mdpi.com/2306-5354/4/1/20PHAmonomer evolutionmixed culturemodelingpolymer compositionbiopolymer
collection DOAJ
language English
format Article
sources DOAJ
author Liliana Montano-Herrera
Bronwyn Laycock
Alan Werker
Steven Pratt
spellingShingle Liliana Montano-Herrera
Bronwyn Laycock
Alan Werker
Steven Pratt
The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents
Bioengineering
PHA
monomer evolution
mixed culture
modeling
polymer composition
biopolymer
author_facet Liliana Montano-Herrera
Bronwyn Laycock
Alan Werker
Steven Pratt
author_sort Liliana Montano-Herrera
title The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents
title_short The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents
title_full The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents
title_fullStr The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents
title_full_unstemmed The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents
title_sort evolution of polymer composition during pha accumulation: the significance of reducing equivalents
publisher MDPI AG
series Bioengineering
issn 2306-5354
publishDate 2017-03-01
description This paper presents a systematic investigation into monomer development during mixed culture Polyhydroxyalkanoates (PHA) accumulation involving concurrent active biomass growth and polymer storage. A series of mixed culture PHA accumulation experiments, using several different substrate-feeding strategies, was carried out. The feedstock comprised volatile fatty acids, which were applied as single carbon sources, as mixtures, or in series, using a fed-batch feed-on-demand controlled bioprocess. A dynamic trend in active biomass growth as well as polymer composition was observed. The observations were consistent over replicate accumulations. Metabolic flux analysis (MFA) was used to investigate metabolic activity through time. It was concluded that carbon flux, and consequently copolymer composition, could be linked with how reducing equivalents are generated.
topic PHA
monomer evolution
mixed culture
modeling
polymer composition
biopolymer
url http://www.mdpi.com/2306-5354/4/1/20
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