Topical green tea formulation with anti-hemorrhagic and antibacterial effects

• Main Authors: Fatemeh Kalalinia, Nafise Amiri, Niloufar Mehrvarzian, Bibi Sedigheh Fazly Bazzaz, Mehrdad Iranshahi, Azadeh Shahroodi, Sepideh Arabzadeh, Mohammadreza Abbaspour, Shapour Badiee Aaval, Jebrail Movaffagh
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2020-08-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: bleeding; green tea; hemostasis; polyvinyl alcohol; tannin; topical formulation
• Online Access: http://ijbms.mums.ac.ir/article_15949_d29d9674f7d64707ee519ef34cd0849b.pdf

<em><strong>Objective(s):</strong></em> Potentially preventable death from uncontrolled hemorrhage clearly indicates the importance of simple, fast and efficient ways to achieving hemostasis. The aim of this study was to develop a topical formulation of green tea extract for reducing bleeding that can be helpful in hemorrhage control.<br /><em><strong>Materials and Methods:</strong></em> Hydroalcoholic extract of green tea was isolated from Camellia sinensis and formulated in polyvinyl alcohol (PVA) to achieve two concentrations of 2% and 4% v/v. Folin-Ciocalteau assay was used to determine the total amount of tannins in extract. Rheological behavior of solutions was investigated by measuring viscosity at shear rates of 0–200 sec−1. Quantitative and qualitative microbial limit tests and minimum inhibitory concentration (MIC) assay were done. The effect of formulations on bleeding time was evaluated in an animal model. <br /><em><strong>Results:</strong></em> The total amount of tannin in green tea extract was 3.8% w/w and addition of green tea significantly increased the viscosity of PVA. The results of MIC assay showed that PVA could not inhibit the growth of bacteria, while, 716 µg/ml of green tea and 2860 µg/ml of green tea/PVA 4% inhibited the growth of Staphylococcus aureus and Pseudomonas aeruginosa. In an animal study both 2% and 4% formulations were able to stop hemorrhage approximately at an equal time compared with tranexamic acid (TXA) 50 mg/ml as a control and the lowest bleeding time was 6.4±0.51 sec for green tea/PVA 4%.<br /><em><strong>Conclusion:</strong></em> Based on our results, the topical formulation of green tea extract in PVA has a great potential for anti-hemorrhage applications.