Uses of direct and indirect immuno-fluorescent techniques for demonstration of nematodes infection in sheep in Nineveh government

• Main Authors: Enas S. Hussein, Sura S. Aghwan
• Format: Article
• Language: Arabic
• Published: University of Mosul, College of Veterinary Medicine 2020-01-01
• Series: Iraqi Journal of Veterinary Sciences
• Subjects: indirect; direct; immunofluorescent; lectin - fluorescein kit
• Online Access: https://vetmedmosul.com/article_163565_49a9f4d256d4c16c4d63aa63ccc666db.pdf

This study was conducted for comparison between two different modern methods for the diagnosis of the nematode worms' infection in sheep we used acridine orange fluorochrome and specific staining kit in fecal samples. We examined 50 sheep fecal samples; the total infection rate was about 96% when was used lectin - fluorescein kit while the total infection rate was 74% when we used acridine orange fluorochrome technique. In conclusion of this study indicated that lectin - fluorescein kit is more effective than acridine orange fluorochrome in the diagnosis of nematode worms' eggs. Also, we conclude from this study the importance of using fluorescence - lectin kit technique in the diagnosis of nematodes eggs because they are distinguished by their speed, efficiency and accuracy, as they compare with the fecal culture technique to cultivate the larvae from eggs in order to diagnosed the type of nematodes eggs, the lectin - fluorescein kit technique is the first in Iraq.