Use of Temporary Immersion System (RITA) in sugarcane the plants propagation by organogenesis

• Main Authors: Laisyn Posada-Pérez, Rafael Gómez-Kosky, Maritza Reyes, Lizvadis Alvares Díaz
• Format: Article
• Language: English
• Published: Universidad Central Marta Abreu de Las Villas 2003-01-01
• Series: Biotecnología Vegetal
• Online Access: https://revista.ibp.co.cu/index.php/BV/article/view/198

The present work had as objective to study the possibilities to use the RITA, in the micropropagation plant via axillary buds of sugarcane and of the banana hybrid (Musa sp. AAAA cv. FHIA-01 and AAAB cv. FHIA-18). Two different culture medium i.e. multiplication and thickenment were studied in the multiplication phase of two varieties of sugarcane. It was also studied the number of initial explants to use (two, four and six), using as control flasks with explants liquid and semisolid medium. The number of immersions in 24 hours was analysed and also the time for culture media change was determined testing two periods, 15 days and 30 days respectively. Similar variables were studied for the case of the banana cultivar except for the medium culture. It was obtained like half of more appropriate cultivation for sugarcane that of multiplication with two explants, in the two used varieties, not presenting significant differences the times of change of half made to the 15 and 30 days. For the case of the banana cultivar employment of five explantes for FHIA-01 and three or five explantes for FHIA-18 to begin the multiplication allowed to reach the best results. Regarding the number of immersions it presented bigger superiority the treatment of three immersions during 24 hours, for both banana cultivars. Keys words: liquid medium, micropropagation, Musa, Saccharum