A T-DNA mutant screen that combines high-throughput phenotyping with the efficient identification of mutated genes by targeted genome sequencing

A T-DNA mutant screen that combines high-throughput phenotyping with the efficient identification of mutated genes by targeted genome sequencing

Abstract Background Nitrogen dioxide (NO2) triggers hypersensitive response (HR)-like cell death in Arabidopsis thaliana. A high-throughput mutant screen was established to identify genes involved in this type of programmed cell death. Results Altogether 14,282 lines of SALK T-DNA insertion mutants...

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Bibliographic Details
Main Authors: Ulrike Frank, Susanne Kublik, Dörte Mayer, Marion Engel, Michael Schloter, Jörg Durner, Frank Gaupels
Format: Article
Language:English
Published: BMC 2019-12-01
Series:BMC Plant Biology
Subjects:
Arabidopsis thaliana
T-DNA insertion
Mutant screen
High-throughput phenotyping
Target enrichment
Next generation sequencing
Online Access:https://doi.org/10.1186/s12870-019-2162-7

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