Clog-free high-throughput microfluidic cell isolation with multifunctional microposts

Abstract Microfluidics have been applied to filtration of rare tumor cells from the blood as liquid biopsies. Processing is highly limited by low flow rates and device clogging due to a single function of fluidic paths. A novel method using multifunctional hybrid functional microposts was developed....

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Main Authors:	Dilip Venugopal, Nanda Kasani, Yariswamy Manjunath, Guangfu Li, Jussuf T. Kaifi, Jae W. Kwon
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-08-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-021-94123-6

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spelling	doaj-17765ab8ef0a4ba5a09651028283e7a52021-08-22T11:23:11ZengNature Publishing GroupScientific Reports2045-23222021-08-011111810.1038/s41598-021-94123-6Clog-free high-throughput microfluidic cell isolation with multifunctional micropostsDilip Venugopal0Nanda Kasani1Yariswamy Manjunath2Guangfu Li3Jussuf T. Kaifi4Jae W. Kwon5Department of Electrical Engineering and Computer Sciences, University of MissouriDepartment of Electrical Engineering and Computer Sciences, University of MissouriDepartment of Surgery, Ellis Fischel Cancer Center, University of MissouriDepartment of Surgery, Ellis Fischel Cancer Center, University of MissouriDepartment of Surgery, Ellis Fischel Cancer Center, University of MissouriDepartment of Electrical Engineering and Computer Sciences, University of MissouriAbstract Microfluidics have been applied to filtration of rare tumor cells from the blood as liquid biopsies. Processing is highly limited by low flow rates and device clogging due to a single function of fluidic paths. A novel method using multifunctional hybrid functional microposts was developed. A swift by-passing route for non-tumor cells was integrated to prevent very common clogging problems. Performance was characterized using microbeads (10 µm) and human cancer cells that were spiked in human blood. Design-I showed a capture efficiency of 96% for microbeads and 87% for cancer cells at 1 ml/min flow rate. An improved Design-II presented a higher capture efficiency of 100% for microbeads and 96% for cancer cells. Our method of utilizing various microfluidic functions of separation, bypass and capture has successfully guaranteed highly efficient separation of rare cells from biological fluids.https://doi.org/10.1038/s41598-021-94123-6
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Dilip Venugopal Nanda Kasani Yariswamy Manjunath Guangfu Li Jussuf T. Kaifi Jae W. Kwon
spellingShingle	Dilip Venugopal Nanda Kasani Yariswamy Manjunath Guangfu Li Jussuf T. Kaifi Jae W. Kwon Clog-free high-throughput microfluidic cell isolation with multifunctional microposts Scientific Reports
author_facet	Dilip Venugopal Nanda Kasani Yariswamy Manjunath Guangfu Li Jussuf T. Kaifi Jae W. Kwon
author_sort	Dilip Venugopal
title	Clog-free high-throughput microfluidic cell isolation with multifunctional microposts
title_short	Clog-free high-throughput microfluidic cell isolation with multifunctional microposts
title_full	Clog-free high-throughput microfluidic cell isolation with multifunctional microposts
title_fullStr	Clog-free high-throughput microfluidic cell isolation with multifunctional microposts
title_full_unstemmed	Clog-free high-throughput microfluidic cell isolation with multifunctional microposts
title_sort	clog-free high-throughput microfluidic cell isolation with multifunctional microposts
publisher	Nature Publishing Group
series	Scientific Reports
issn	2045-2322
publishDate	2021-08-01
description	Abstract Microfluidics have been applied to filtration of rare tumor cells from the blood as liquid biopsies. Processing is highly limited by low flow rates and device clogging due to a single function of fluidic paths. A novel method using multifunctional hybrid functional microposts was developed. A swift by-passing route for non-tumor cells was integrated to prevent very common clogging problems. Performance was characterized using microbeads (10 µm) and human cancer cells that were spiked in human blood. Design-I showed a capture efficiency of 96% for microbeads and 87% for cancer cells at 1 ml/min flow rate. An improved Design-II presented a higher capture efficiency of 100% for microbeads and 96% for cancer cells. Our method of utilizing various microfluidic functions of separation, bypass and capture has successfully guaranteed highly efficient separation of rare cells from biological fluids.
url	https://doi.org/10.1038/s41598-021-94123-6
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Clog-free high-throughput microfluidic cell isolation with multifunctional microposts

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