Anti-lamin A/C antibodies in patients with Behçet’s disease

• Main Authors: Peng Chen, Hai Yan, Wen Zhang, Chunhe Yang, Hongwu Du
• Format: Article
• Language: English
• Published: Termedia Publishing House 2018-10-01
• Series: Central European Journal of Immunology
• Subjects: Behçet’s disease; autoantigen; lamin A/C
• Online Access: https://www.termedia.pl/Anti-lamin-A-C-antibodies-in-patients-with-Behcet-s-disease,10,34300,1,1.html
• ISSN: 1426-3912; 1644-4124

Lamin A/C has been identified as a target antigen of anti-endothelial cell antibodies in vasculitis. Behçet’s disease (BD) is a chronic vasculitis with unclear pathogenesis. Self-antibodies have been found to play an important role in the pathological process of BD. The aim of this study was to verify whether lamin A/C is an autoantigen of BD. First, the human lamin A/C protein was cloned and purified and verified by Western blotting. Next, a homemade ELISA kit was used to test the presence of anti-lamin A/C antibodies in BD patients. This is the first time it has been certified that lamin A/C is an autoantigen of BD. The reactivity of serum IgG against recombinant human lamin A/C was detected in 7 of 31 BD patients (23%) and 3 of 32 healthy controls (HC) (9%).