Collective Migration of Lens Epithelial Cell Induced by Differential Microscale Groove Patterns
Herein, a micro-patterned cell adhesive surface is prepared for the future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro patterns were prepared by a photolithography process. We investigated the effect of microscale topographical patterned surfaces on decreasing the co...
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doaj-1735d64d3f6146aba2e59127115cc5dc2020-11-24T23:08:34ZengMDPI AGJournal of Functional Biomaterials2079-49832017-08-01833410.3390/jfb8030034jfb8030034Collective Migration of Lens Epithelial Cell Induced by Differential Microscale Groove PatternsChunga Kwon0Youngjun Kim1Hojeong Jeon2Korea Institute of Science and Technology Europe (KIST-Europe) Forschungsgesellschaft mbH, Campus E 7 1, 66123 Saarbrücken, GermanyKorea Institute of Science and Technology Europe (KIST-Europe) Forschungsgesellschaft mbH, Campus E 7 1, 66123 Saarbrücken, GermanyCenter for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, KoreaHerein, a micro-patterned cell adhesive surface is prepared for the future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro patterns were prepared by a photolithography process. We investigated the effect of microscale topographical patterned surfaces on decreasing the collective cell migration rate. PDMS substrates were prepared through soft lithography using Si molds fabricated by photolithography. Afterwards, we observed the collective cell migration of human lens epithelial cells (B-3) on various groove/ridge patterns and evaluated the migration rate to determine the pattern most effective in slowing down the cell sheet spreading speed. Microgroove patterns were variable, with widths of 3, 5, and 10 µm. After the seeding, time-lapse images were taken under controlled cell culturing conditions. Cell sheet borders were drawn in order to assess collective migration rate. Our experiments revealed that the topographical patterned surfaces could be applied to intraocular lenses to prevent or slow the development of posterior capsular opacification (PCO) by delaying the growth and spread of human lens epithelial cells.https://www.mdpi.com/2079-4983/8/3/34polydimethylsiloxane (PDMS)micro-patternshuman lens epithelial cells (B-3)microgroove patternsposterior capsular opacification (PCO) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chunga Kwon Youngjun Kim Hojeong Jeon |
spellingShingle |
Chunga Kwon Youngjun Kim Hojeong Jeon Collective Migration of Lens Epithelial Cell Induced by Differential Microscale Groove Patterns Journal of Functional Biomaterials polydimethylsiloxane (PDMS) micro-patterns human lens epithelial cells (B-3) microgroove patterns posterior capsular opacification (PCO) |
author_facet |
Chunga Kwon Youngjun Kim Hojeong Jeon |
author_sort |
Chunga Kwon |
title |
Collective Migration of Lens Epithelial Cell Induced by Differential Microscale Groove Patterns |
title_short |
Collective Migration of Lens Epithelial Cell Induced by Differential Microscale Groove Patterns |
title_full |
Collective Migration of Lens Epithelial Cell Induced by Differential Microscale Groove Patterns |
title_fullStr |
Collective Migration of Lens Epithelial Cell Induced by Differential Microscale Groove Patterns |
title_full_unstemmed |
Collective Migration of Lens Epithelial Cell Induced by Differential Microscale Groove Patterns |
title_sort |
collective migration of lens epithelial cell induced by differential microscale groove patterns |
publisher |
MDPI AG |
series |
Journal of Functional Biomaterials |
issn |
2079-4983 |
publishDate |
2017-08-01 |
description |
Herein, a micro-patterned cell adhesive surface is prepared for the future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro patterns were prepared by a photolithography process. We investigated the effect of microscale topographical patterned surfaces on decreasing the collective cell migration rate. PDMS substrates were prepared through soft lithography using Si molds fabricated by photolithography. Afterwards, we observed the collective cell migration of human lens epithelial cells (B-3) on various groove/ridge patterns and evaluated the migration rate to determine the pattern most effective in slowing down the cell sheet spreading speed. Microgroove patterns were variable, with widths of 3, 5, and 10 µm. After the seeding, time-lapse images were taken under controlled cell culturing conditions. Cell sheet borders were drawn in order to assess collective migration rate. Our experiments revealed that the topographical patterned surfaces could be applied to intraocular lenses to prevent or slow the development of posterior capsular opacification (PCO) by delaying the growth and spread of human lens epithelial cells. |
topic |
polydimethylsiloxane (PDMS) micro-patterns human lens epithelial cells (B-3) microgroove patterns posterior capsular opacification (PCO) |
url |
https://www.mdpi.com/2079-4983/8/3/34 |
work_keys_str_mv |
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