The draft genome of the blood pheasant (Ithaginis cruentus): Phylogeny and high‐altitude adaptation

• Main Authors: Chuang Zhou, Yi Liu, Lu Qiao, Yang Liu, Nan Yang, Yang Meng, Bisong Yue
• Format: Article
• Language: English
• Published: Wiley 2020-10-01
• Series: Ecology and Evolution
• Subjects: blood pheasant; comparative genomics; high‐altitude adaptation; missense mutation; phylogenetic analysis; positive selection
• Online Access: https://doi.org/10.1002/ece3.6782

Abstract The blood pheasant (Ithaginis cruentus), the only species in the genus Ithaginis, lives in an extremely inhospitable high‐altitude environment, coping with hypoxia and ultraviolet (UV) radiation. To further investigate the phylogeny of Phasianidae species based on complete genomes and understand the molecular genetic mechanisms of the high‐altitude adaptation of the blood pheasant, we de novo assembled and annotated the complete genome of the blood pheasant. The blood pheasant genome size is 1.04 Gb with scaffold N50 of 10.88 Mb. We identified 109.92 Mb (10.62%) repetitive elements, 279,037 perfect microsatellites, and 17,209 protein‐coding genes. The phylogenetic tree of Phasianidae based on whole genomes revealed three highly supported major clades with the blood pheasant included in the “erectile clade.” Comparative genomics analysis showed that many genes were positively selected in the blood pheasant, which was associated with response to hypoxia and/or UV radiation. More importantly, among these positively selected genes (PSGs) which were related to high‐altitude adaptation, sixteen PSGs had blood pheasant‐specific missense mutations. Our data and analysis lay solid foundation to the study of Phasianidae phylogeny and provided new insights into the potential adaptation mechanisms to the high altitude employed by the blood pheasant.