Real-time monitoring of T-cell-secreted interferon-γ for the diagnosis of tuberculosis

• Main Authors: Changlin Wu, Jianan He, Boan Li, YunQing Xu, Dayong Gu, Houming Liu, Dan Zhao, Chaopeng Shao
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2018-07-01
• Series: Biotechnology & Biotechnological Equipment
• Subjects: Surface plasmon resonance; protein microarray; tubercle bacillus; CD4+ T-cells; IFN-γ; label-free
• Online Access: http://dx.doi.org/10.1080/13102818.2018.1432416

In this paper, we report the development of a label-free biosensor, based on surface plasmon resonance, for real-time monitoring of captured human CD4+T-cells, and their dynamic cytokine production upon specific antigen stimulation. Microarrays of CD4+T-cells, and interferon gamma (IFN-γ) specific antibody (Ab) spots were printed side by side onto the poly(OEGMA-co-HEMA) matrix. The placement of CD4+ T-cells near the anti IFN-γ Ab-coated spots ensured a high local concentration of secreted IFN-γ for detection. We have demonstrated that this approach enables the detection of tuberculosis (TB) infection in clinical samples, with an overall sensitivity of 85.5% and an overall specificity of 97.7%.