Recombinational branch migration by the RadA/Sms paralog of RecA in Escherichia coli

• Main Authors: Deani L Cooper, Susan T Lovett
• Format: Article
• Language: English
• Published: eLife Sciences Publications Ltd 2016-02-01
• Series: eLife
• Subjects: DNA repair; DNA damage response; double-strand break repair; DNA mutagenesis; homologous recombination
• Online Access: https://elifesciences.org/articles/10807

RadA (also known as 'Sms') is a highly conserved protein, found in almost all eubacteria and plants, with sequence similarity to the RecA strand exchange protein and a role in homologous recombination. We investigate here the biochemical properties of the E. coli RadA protein and several mutant forms. RadA is a DNA-dependent ATPase, a DNA-binding protein and can stimulate the branch migration phase of RecA-mediated strand transfer reactions. RadA cannot mediate synaptic pairing between homologous DNA molecules but can drive branch migration to extend the region of heteroduplex DNA, even without RecA. Unlike other branch migration factors RecG and RuvAB, RadA stimulates branch migration within the context of the RecA filament, in the direction of RecA-mediated strand exchange. We propose that RadA-mediated branch migration aids recombination by allowing the 3’ invading strand to be incorporated into heteroduplex DNA and to be extended by DNA polymerases.