Improved expression of Catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coli

Catechol 1,2-dioxygenase (CAT1) is a key enzyme for the ortho-cleavage pathway involved in the degradation of dibenzofuran, a dioxin derivative, which is a highly toxic environmental pollutant. The present study aims to investigate appropriate culture conditions for enhancing the expression of the c...

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Main Authors:	Van Thanh Dang, Anh Thi Hoang, Gia Cat Tuong Tran, Thi Huyen Tran Pham, Le Thi Ha Thanh, Hoang Loc Nguyen
Format:	Article
Language:	English
Published:	HUJOS 2020-11-01
Series:	Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên
Subjects:	escherichia coli m15 cat1 gene expression dibenzofuran
Online Access:	http://jos.hueuni.edu.vn/index.php/HUJOS-NS/article/view/5974

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spelling	doaj-1668ca4993814024917749b2e4a215b52021-01-18T10:33:07ZengHUJOSTạp chí Khoa học Đại học Huế: Khoa học Tự nhiên1859-13882615-96782020-11-011291D616510.26459/hueunijns.v129i1D.59748204Improved expression of Catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coliVan Thanh Dang0Anh Thi Hoang1Gia Cat Tuong Tran2Thi Huyen Tran Pham3Le Thi Ha Thanh4Hoang Loc Nguyen5Department of Biotechnology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, VietnamDepartment of Biotechnology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, VietnamDepartment of Biotechnology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, VietnamDepartment of Biotechnology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, VietnamDepartment of Biotechnology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, VietnamDepartment of Biotechnology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, VietnamCatechol 1,2-dioxygenase (CAT1) is a key enzyme for the ortho-cleavage pathway involved in the degradation of dibenzofuran, a dioxin derivative, which is a highly toxic environmental pollutant. The present study aims to investigate appropriate culture conditions for enhancing the expression of the cat1 gene encoding CAT1 enzyme from Burkholderia cepacia DF4 in Escherichia coli M15. The optimized culture conditions for gene expression are cell density at the time of induction, shaking speed, induction temperature, induction time, and inducer concentration. The highest level for CAT1 was obtained at the IPTG concentration of 1.2 mM, 10 hours after induction at 35 °C, shaking speed 200 rpm with cell density at OD600 0.7.http://jos.hueuni.edu.vn/index.php/HUJOS-NS/article/view/5974escherichia coli m15cat1gene expressiondibenzofuran
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Van Thanh Dang Anh Thi Hoang Gia Cat Tuong Tran Thi Huyen Tran Pham Le Thi Ha Thanh Hoang Loc Nguyen
spellingShingle	Van Thanh Dang Anh Thi Hoang Gia Cat Tuong Tran Thi Huyen Tran Pham Le Thi Ha Thanh Hoang Loc Nguyen Improved expression of Catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coli Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên escherichia coli m15 cat1 gene expression dibenzofuran
author_facet	Van Thanh Dang Anh Thi Hoang Gia Cat Tuong Tran Thi Huyen Tran Pham Le Thi Ha Thanh Hoang Loc Nguyen
author_sort	Van Thanh Dang
title	Improved expression of Catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coli
title_short	Improved expression of Catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coli
title_full	Improved expression of Catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coli
title_fullStr	Improved expression of Catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coli
title_full_unstemmed	Improved expression of Catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coli
title_sort	improved expression of catechol 1,2-dioxygenase gene from burkholderia cepacia in escherichia coli
publisher	HUJOS
series	Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên
issn	1859-1388 2615-9678
publishDate	2020-11-01
description	Catechol 1,2-dioxygenase (CAT1) is a key enzyme for the ortho-cleavage pathway involved in the degradation of dibenzofuran, a dioxin derivative, which is a highly toxic environmental pollutant. The present study aims to investigate appropriate culture conditions for enhancing the expression of the cat1 gene encoding CAT1 enzyme from Burkholderia cepacia DF4 in Escherichia coli M15. The optimized culture conditions for gene expression are cell density at the time of induction, shaking speed, induction temperature, induction time, and inducer concentration. The highest level for CAT1 was obtained at the IPTG concentration of 1.2 mM, 10 hours after induction at 35 °C, shaking speed 200 rpm with cell density at OD600 0.7.
topic	escherichia coli m15 cat1 gene expression dibenzofuran
url	http://jos.hueuni.edu.vn/index.php/HUJOS-NS/article/view/5974
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Improved expression of Catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coli

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