Expression of hypoxia ⁃inducible factor 1α in the pressure ⁃side periodontium during orthodontic tooth move⁃ ment in rats

• Main Authors: HE Zhengquan, YANG Kai
• Format: Article
• Language: zho
• Published: Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases 2018-04-01
• Series: 口腔疾病防治
• Subjects: Orthodontics; Tooth movement; Periodontal tissue remodeling; Hypoxia; Inducible factor 1α
• Online Access: http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2018.04.005
• ISSN: 2096-1456; 2096-1456

Objective To assess hypoxia⁃inducible factor 1α (HIF⁃1α) expression levels in the periodontal tissues of the pressure side during orthodontic tooth movement in rats. Methods A total of 50 male 6⁃week⁃old Sprague Daw⁃ ley (SD) rats were randomly divided into 10 groups of 5 rats each. The upper left first molar was the experimental tooth and was pulled mesially with an orthodontic force of 10⁃15 g for 0, 1, 3, 6, or 12 h, or 1, 3, 7, 14, or 21 d. Routine five⁃ micrometer paraffin⁃embedded tissue sections were processed for HE staining and immunohistochemical staining for HIF ⁃1α. The Image⁃Pro Plus system was used to quantitatively analyze the stained slices. Results The expression of HIF⁃ 1α in the periodontal tissue of the pressure side changed during the process of orthodontic tooth movement. The expres⁃ sion of HIF⁃1α increased immediately after loading for 1 h, reached a small peak at 3 h, and then decreased. After 12 h, the expression increased again, reached a peak after 1 d, and then gradually decreased to near the pre⁃loading level (P < 0.05). Conclusion There were differences in expression of HIF⁃1α in different groups in the periodontal tissues of the pressure side during orthodontic tooth movement in rats.