Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging
Abstract Using two-dimensional top-down view microscopy, researchers have recently described chondrocytes as being spatially arranged in distinct patterns such as strings, double strings, and small and large clusters. Because of the seeming association of these changes with tissue degeneration, they...
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2021-05-01
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doaj-1639485ef297488cbcc2c013107d06602021-05-09T11:34:02ZengNature Publishing GroupScientific Reports2045-23222021-05-0111111110.1038/s41598-021-89582-wExploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imagingMarina Danalache0Kevin Ralf Beutler1Bernd Rolauffs2Julius Michael Wolfgart3Florian Christof Bonnaire4Stefan Fischer5Imke Greving6Ulf Krister Hofmann7Department of Orthopaedic Surgery, University Hospital of TübingenMedical Faculty of the University of TübingenG.E.R.N. Tissue Replacement, Regeneration and Neogenesis, Department of Orthopedics and Trauma Surgery, Medical Center - Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of FreiburgMedical Faculty of the University of TübingenDepartment of Orthopaedic Surgery, University Hospital of TübingenDepartment of Evolutionary Biology of Invertebrates, University of TübingenInstitute of Materials Research, Helmholtz-Zentrum GeesthachtDepartment of Orthopaedic Surgery, University Hospital of TübingenAbstract Using two-dimensional top-down view microscopy, researchers have recently described chondrocytes as being spatially arranged in distinct patterns such as strings, double strings, and small and large clusters. Because of the seeming association of these changes with tissue degeneration, they have been proposed as an image-based biomarker for early osteoarthritis (OA) staging. The aim of our study was to investigate the spatial arrangement of chondrocytes in human articular cartilage in a 3D fashion and to evaluate the 3D changes of these patterns in the context of local tissue destruction. Decalcified femoral condyle resections from the load-bearing area were analysed in 3D for their spatial chondrocyte organisation by means of fluorescence microscopy and synchrotron-radiation micro-computed tomography (SR-µCT). In intact cartilage chondrocyte strings can be found in the superficial, transitional and deep zones. The proposed pattern changes accompanying tissue destruction could be located not just along the surface but also through all layers of cartilage. Each spatial pattern was characterised by a different cellular density (the only exception being between single and double strings with p = 0.062), with cellular density significantly increasing alongside the increase in local tissue degeneration as defined by the chondrocyte patterns. We can thus corroborate that the proposed cellular spatial changes are a three-dimensional function of local tissue degeneration, underlining their relevance as an image-based biomarker for the early diagnosis and description of OA. Clinical trial registration number: Project number of the ethics committee of the University of Tübingen:171/2014BO2.https://doi.org/10.1038/s41598-021-89582-w |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marina Danalache Kevin Ralf Beutler Bernd Rolauffs Julius Michael Wolfgart Florian Christof Bonnaire Stefan Fischer Imke Greving Ulf Krister Hofmann |
spellingShingle |
Marina Danalache Kevin Ralf Beutler Bernd Rolauffs Julius Michael Wolfgart Florian Christof Bonnaire Stefan Fischer Imke Greving Ulf Krister Hofmann Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging Scientific Reports |
author_facet |
Marina Danalache Kevin Ralf Beutler Bernd Rolauffs Julius Michael Wolfgart Florian Christof Bonnaire Stefan Fischer Imke Greving Ulf Krister Hofmann |
author_sort |
Marina Danalache |
title |
Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging |
title_short |
Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging |
title_full |
Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging |
title_fullStr |
Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging |
title_full_unstemmed |
Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging |
title_sort |
exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3d imaging |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-05-01 |
description |
Abstract Using two-dimensional top-down view microscopy, researchers have recently described chondrocytes as being spatially arranged in distinct patterns such as strings, double strings, and small and large clusters. Because of the seeming association of these changes with tissue degeneration, they have been proposed as an image-based biomarker for early osteoarthritis (OA) staging. The aim of our study was to investigate the spatial arrangement of chondrocytes in human articular cartilage in a 3D fashion and to evaluate the 3D changes of these patterns in the context of local tissue destruction. Decalcified femoral condyle resections from the load-bearing area were analysed in 3D for their spatial chondrocyte organisation by means of fluorescence microscopy and synchrotron-radiation micro-computed tomography (SR-µCT). In intact cartilage chondrocyte strings can be found in the superficial, transitional and deep zones. The proposed pattern changes accompanying tissue destruction could be located not just along the surface but also through all layers of cartilage. Each spatial pattern was characterised by a different cellular density (the only exception being between single and double strings with p = 0.062), with cellular density significantly increasing alongside the increase in local tissue degeneration as defined by the chondrocyte patterns. We can thus corroborate that the proposed cellular spatial changes are a three-dimensional function of local tissue degeneration, underlining their relevance as an image-based biomarker for the early diagnosis and description of OA. Clinical trial registration number: Project number of the ethics committee of the University of Tübingen:171/2014BO2. |
url |
https://doi.org/10.1038/s41598-021-89582-w |
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