Effect of Overexpression of Follistatin-like Protein 1 on Downstream Genes in Cervical Cancer and Bioinformatic Analysis of Downstream Genes
Objective To investigate the regulation mechanism of follistatin-like protein 1 (FSTL1) in cervical cancer by gene chip technology to analyze the changes of downstream genes and signaling pathways of FSTL1. Methods The differentially-expressed genes were screened by gene chip technology in HeLa cell...
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Magazine House of Cancer Research on Prevention and Treatment
2019-01-01
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doaj-162d88fc3e2e4f34a50eccdb3d3087a12020-11-25T03:16:53ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782019-01-01461263110.3971/j.issn.1000-8578.2019.18.06658578.2019.18.0665Effect of Overexpression of Follistatin-like Protein 1 on Downstream Genes in Cervical Cancer and Bioinformatic Analysis of Downstream GenesZHANG Hong0LUO Ruoyu1HU Xiaoxia2CHEN Huan3WANG Linlin4Department of Gynecology, Renmin Hospital of Wuhan University, Wuhan 430000, ChinaDepartment of Gynecology, Renmin Hospital of Wuhan University, Wuhan 430000, ChinaDepartment of Gynecology, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, ChinaDepartment of Gynecology, Renmin Hospital of Wuhan University, Wuhan 430000, ChinaDepartment of Gynecology, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, ChinaObjective To investigate the regulation mechanism of follistatin-like protein 1 (FSTL1) in cervical cancer by gene chip technology to analyze the changes of downstream genes and signaling pathways of FSTL1. Methods The differentially-expressed genes were screened by gene chip technology in HeLa cells transfected with FSTL1. The differentially-expressed genes and regulation mechanism of FSTL1 in cervical cancer were found by bioinformatics analysis. The relative expression of 29 differentially-expressed genes were verified by real-time quantitative polymerase chain reaction (RT-qPCR). Results Compared with the negative control group, there were 881 differentially-expressed genes in the experimental group. These differentially-expressed genes mainly enriched in the biological processes of transcription regulation, cell proliferation, apoptosis and adhesion, as well as transcription misregulation of cancer, MAPK and P53 signal pathway. RT-qPCR results showed that FSTL1 up-regulated the relevant factors of PI3K/AKT/Bax/Bcl-2 and P53 signaling pathway, which was basically consistent with the results of gene chip. Conclusion The differentially-expressed genes of FSTL1 enrich in the biological processes of transcription regulation, cell proliferation, apoptosis and adhesion and may participate in the occurrence and development of cervical cancer by regulating PI3K/AKT/Bax/Bcl-2 and P53 signaling pathways.http://html.rhhz.net/ZLFZYJ/html/8578.2019.18.0665.htmcervical cancerfstl1gene chipsbioinformaticsrt-qpcr |
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language |
zho |
format |
Article |
sources |
DOAJ |
author |
ZHANG Hong LUO Ruoyu HU Xiaoxia CHEN Huan WANG Linlin |
spellingShingle |
ZHANG Hong LUO Ruoyu HU Xiaoxia CHEN Huan WANG Linlin Effect of Overexpression of Follistatin-like Protein 1 on Downstream Genes in Cervical Cancer and Bioinformatic Analysis of Downstream Genes Zhongliu Fangzhi Yanjiu cervical cancer fstl1 gene chips bioinformatics rt-qpcr |
author_facet |
ZHANG Hong LUO Ruoyu HU Xiaoxia CHEN Huan WANG Linlin |
author_sort |
ZHANG Hong |
title |
Effect of Overexpression of Follistatin-like Protein 1 on Downstream Genes in Cervical Cancer and Bioinformatic Analysis of Downstream Genes |
title_short |
Effect of Overexpression of Follistatin-like Protein 1 on Downstream Genes in Cervical Cancer and Bioinformatic Analysis of Downstream Genes |
title_full |
Effect of Overexpression of Follistatin-like Protein 1 on Downstream Genes in Cervical Cancer and Bioinformatic Analysis of Downstream Genes |
title_fullStr |
Effect of Overexpression of Follistatin-like Protein 1 on Downstream Genes in Cervical Cancer and Bioinformatic Analysis of Downstream Genes |
title_full_unstemmed |
Effect of Overexpression of Follistatin-like Protein 1 on Downstream Genes in Cervical Cancer and Bioinformatic Analysis of Downstream Genes |
title_sort |
effect of overexpression of follistatin-like protein 1 on downstream genes in cervical cancer and bioinformatic analysis of downstream genes |
publisher |
Magazine House of Cancer Research on Prevention and Treatment |
series |
Zhongliu Fangzhi Yanjiu |
issn |
1000-8578 1000-8578 |
publishDate |
2019-01-01 |
description |
Objective To investigate the regulation mechanism of follistatin-like protein 1 (FSTL1) in cervical cancer by gene chip technology to analyze the changes of downstream genes and signaling pathways of FSTL1. Methods The differentially-expressed genes were screened by gene chip technology in HeLa cells transfected with FSTL1. The differentially-expressed genes and regulation mechanism of FSTL1 in cervical cancer were found by bioinformatics analysis. The relative expression of 29 differentially-expressed genes were verified by real-time quantitative polymerase chain reaction (RT-qPCR). Results Compared with the negative control group, there were 881 differentially-expressed genes in the experimental group. These differentially-expressed genes mainly enriched in the biological processes of transcription regulation, cell proliferation, apoptosis and adhesion, as well as transcription misregulation of cancer, MAPK and P53 signal pathway. RT-qPCR results showed that FSTL1 up-regulated the relevant factors of PI3K/AKT/Bax/Bcl-2 and P53 signaling pathway, which was basically consistent with the results of gene chip. Conclusion The differentially-expressed genes of FSTL1 enrich in the biological processes of transcription regulation, cell proliferation, apoptosis and adhesion and may participate in the occurrence and development of cervical cancer by regulating PI3K/AKT/Bax/Bcl-2 and P53 signaling pathways. |
topic |
cervical cancer fstl1 gene chips bioinformatics rt-qpcr |
url |
http://html.rhhz.net/ZLFZYJ/html/8578.2019.18.0665.htm |
work_keys_str_mv |
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