Examining a DNA Replication Requirement for Bacteriophage λ Red- and Rac Prophage RecET-Promoted Recombination in Escherichia coli

Examining a DNA Replication Requirement for Bacteriophage λ Red- and Rac Prophage RecET-Promoted Recombination in Escherichia coli

Recombineering, in vivo genetic engineering with bacteriophage homologous recombination systems, is a powerful technique for making genetic modifications in bacteria. Two systems widely used in Escherichia coli are the Red system from phage λ and RecET from the defective Rac prophage. We investigate...

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Bibliographic Details
Main Authors: Lynn C. Thomason, Nina Costantino, Donald L. Court
Format: Article
Language:English
Published: American Society for Microbiology 2016-09-01
Series:mBio
Online Access:http://mbio.asm.org/cgi/content/full/7/5/e01443-16

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