Study on Quantitative Phase Imaging by Dual-Wavelength Digital Holography Microscopy

Study on Quantitative Phase Imaging by Dual-Wavelength Digital Holography Microscopy

A dual-wavelength digital holographic microscopy with premagnification is proposed to obtain the object surface measurements over the large gradient. The quantitative phase images of specimens are captured in high precision by the processing of filtering and phase compensation. The phase images are...

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Main Authors: Lin Hu, Yunxiu Shui, Yaohui Dai, Haiyu Wu, Gang Zhu, Yan Yang
Format: Article
Language:English
Published: Hindawi Limited 2018-01-01
Series:International Journal of Optics
Online Access:http://dx.doi.org/10.1155/2018/2312137
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spelling doaj-15fbca8ecc654639b079356f7507cf6b2020-11-25T00:17:05ZengHindawi LimitedInternational Journal of Optics1687-93841687-93922018-01-01201810.1155/2018/23121372312137Study on Quantitative Phase Imaging by Dual-Wavelength Digital Holography MicroscopyLin Hu0Yunxiu Shui1Yaohui Dai2Haiyu Wu3Gang Zhu4Yan Yang5College of Mechanical Engineering, Chongqing University of Technology, Chongqing 400054, ChinaCollege of Mechanical Engineering, Chongqing University of Technology, Chongqing 400054, ChinaCollege of Mechanical Engineering, Chongqing University of Technology, Chongqing 400054, ChinaCollege of Mechanical Engineering, Chongqing University of Technology, Chongqing 400054, ChinaCollege of Mechanical Engineering, Chongqing University of Technology, Chongqing 400054, ChinaCollege of Mechanical Engineering, Chongqing University of Technology, Chongqing 400054, ChinaA dual-wavelength digital holographic microscopy with premagnification is proposed to obtain the object surface measurements over the large gradient. The quantitative phase images of specimens are captured in high precision by the processing of filtering and phase compensation. The phase images are acquired without phase unwrapping, which is necessary in traditional digital holographic microscopy; thereby the proposed system can greatly increase the speed of reconstruction. The results of numerical simulation and optical experiments demonstrated that the reconstructed speed increased by 37.9 times, and the relative error of measurement is 4% compared with the traditional holographic microscopy system. It means that the proposed system can directly acquire the higher quality quantitative phase distribution for specimens.http://dx.doi.org/10.1155/2018/2312137
collection DOAJ
language English
format Article
sources DOAJ
author Lin Hu
Yunxiu Shui
Yaohui Dai
Haiyu Wu
Gang Zhu
Yan Yang
spellingShingle Lin Hu
Yunxiu Shui
Yaohui Dai
Haiyu Wu
Gang Zhu
Yan Yang
Study on Quantitative Phase Imaging by Dual-Wavelength Digital Holography Microscopy
International Journal of Optics
author_facet Lin Hu
Yunxiu Shui
Yaohui Dai
Haiyu Wu
Gang Zhu
Yan Yang
author_sort Lin Hu
title Study on Quantitative Phase Imaging by Dual-Wavelength Digital Holography Microscopy
title_short Study on Quantitative Phase Imaging by Dual-Wavelength Digital Holography Microscopy
title_full Study on Quantitative Phase Imaging by Dual-Wavelength Digital Holography Microscopy
title_fullStr Study on Quantitative Phase Imaging by Dual-Wavelength Digital Holography Microscopy
title_full_unstemmed Study on Quantitative Phase Imaging by Dual-Wavelength Digital Holography Microscopy
title_sort study on quantitative phase imaging by dual-wavelength digital holography microscopy
publisher Hindawi Limited
series International Journal of Optics
issn 1687-9384
1687-9392
publishDate 2018-01-01
description A dual-wavelength digital holographic microscopy with premagnification is proposed to obtain the object surface measurements over the large gradient. The quantitative phase images of specimens are captured in high precision by the processing of filtering and phase compensation. The phase images are acquired without phase unwrapping, which is necessary in traditional digital holographic microscopy; thereby the proposed system can greatly increase the speed of reconstruction. The results of numerical simulation and optical experiments demonstrated that the reconstructed speed increased by 37.9 times, and the relative error of measurement is 4% compared with the traditional holographic microscopy system. It means that the proposed system can directly acquire the higher quality quantitative phase distribution for specimens.
url http://dx.doi.org/10.1155/2018/2312137
work_keys_str_mv AT linhu studyonquantitativephaseimagingbydualwavelengthdigitalholographymicroscopy
AT yunxiushui studyonquantitativephaseimagingbydualwavelengthdigitalholographymicroscopy
AT yaohuidai studyonquantitativephaseimagingbydualwavelengthdigitalholographymicroscopy
AT haiyuwu studyonquantitativephaseimagingbydualwavelengthdigitalholographymicroscopy
AT gangzhu studyonquantitativephaseimagingbydualwavelengthdigitalholographymicroscopy
AT yanyang studyonquantitativephaseimagingbydualwavelengthdigitalholographymicroscopy
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