Genic Variant Detection of Caspian Horse Using High-throughput Sequencing Technology

• Main Authors: Babak Aref Nezhad, Hamid Kahram, Mohammad Moradi, Malek Shaekri, yang Dong, Khiaouli Zhang, Van Vang, Qasem Hosseini
• Format: Article
• Language: fas
• Published: Shahid Bahonar University of Kerman 2015-02-01
• Series: مجله بیوتکنولوژی کشاورزی
• Subjects: high-throughput sequencing; caspian horse; genic variants; biological pathways
• Online Access: https://jab.uk.ac.ir/article_1343_a18ac291149a50ca0ed96346f9e54192.pdf
• ISSN: 2228-6705; 2228-6500

Recently, new advanced high-throughput sequencing technology as a novel tool has opened the way to study of genomic variants and functional information stored within farm animals. The Caspian horse is one of the valuable horses ever exist in the world. Hence, propose of this study was to investigate genetic variants of single nucleotide polymorphisms, insertion and deletions and copy number variations within the genome of Caspian horse and their involved biological pathways. Using high-throughput sequencing technology, we generated 108 Gb (Average depth of 45.8) of DNA sequence from three Caspian horse mares resulting in an average of 14.41X coverage and 76.4% covered with reference genome. Using a stringent filtering method, we identified 1666717 single nucleotide polymorphisms, 358020 insertion and deletions, and 3109 copy number variations. Functional clustering analysis of genic variants revealed that most of the genetic variants in the Caspian horse’s genome were enriched in nervous system, GTP-related signal transduction, cellular morphogenesis, cytoskeleton organization, vascular development and cellular movement. Moreover, we have detected structural variations as like as inversion, intra- and inter-chromosomal translocations, large insertion and deletions which could be useful for marker based population genetic investigation