Neuronal modulation of brown adipose activity through perturbation of white adipocyte lipogenesis

Neuronal modulation of brown adipose activity through perturbation of white adipocyte lipogenesis

Objective: Crosstalk between adipocytes and local neurons may be an important regulatory mechanism to control energy homeostasis. We previously reported that perturbation of adipocyte de novo lipogenesis (DNL) by deletion of fatty acid synthase (FASN) expands sympathetic neurons within white adipose...

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Main Authors: Adilson Guilherme, David J. Pedersen, Felipe Henriques, Alexander H. Bedard, Elizabeth Henchey, Mark Kelly, Donald A. Morgan, Kamal Rahmouni, Michael P. Czech
Format: Article
Language:English
Published: Elsevier 2018-10-01
Series:Molecular Metabolism
Online Access:http://www.sciencedirect.com/science/article/pii/S2212877818305180
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spelling doaj-15917499c6d14b8da7c28dcc88f2cde22020-11-24T22:24:33ZengElsevierMolecular Metabolism2212-87782018-10-0116116125Neuronal modulation of brown adipose activity through perturbation of white adipocyte lipogenesisAdilson Guilherme0David J. Pedersen1Felipe Henriques2Alexander H. Bedard3Elizabeth Henchey4Mark Kelly5Donald A. Morgan6Kamal Rahmouni7Michael P. Czech8Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USAProgram in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USAProgram in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USAProgram in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USAProgram in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USAProgram in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USADepartment of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USADepartment of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USAProgram in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USA; Corresponding author. Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Biotech 2, Suite 100, Worcester, MA, 01605, USA. Fax: +1 508 856 1617.Objective: Crosstalk between adipocytes and local neurons may be an important regulatory mechanism to control energy homeostasis. We previously reported that perturbation of adipocyte de novo lipogenesis (DNL) by deletion of fatty acid synthase (FASN) expands sympathetic neurons within white adipose tissue (WAT) and stimulates the appearance of “beige” adipocytes. Here we tested whether WAT DNL activity can also influence neuronal regulation and thermogenesis in brown adipose tissue (BAT). Methods and results: Induced deletion of FASN in all adipocytes in mature mice (iAdFASNKO) enhanced sympathetic innervation and neuronal activity as well as UCP1 expression in both WAT and BAT. This increased sympathetic innervation could be observed at both 22 °C and 30 °C, indicating it is not a response to heat loss but rather adipocyte signaling. In contrast, selective ablation of FASN in brown adipocytes of mice (iUCP1FASNKO) failed to modulate sympathetic innervation and the thermogenic program in BAT. Surprisingly, DNL in brown adipocytes was also dispensable in maintaining euthermia when UCP1FASNKO mice were cold-exposed. Conclusion: These results indicate that DNL in white adipocytes influences long distance signaling to BAT, which can modify BAT sympathetic innervation and expression of genes involved in thermogenesis. Keywords: Adipocytes, Lipogenesis, Brown adipose tissue, Thermogenesis, Sensory nerve, Sympathetic nerve, SNS outflowhttp://www.sciencedirect.com/science/article/pii/S2212877818305180
collection DOAJ
language English
format Article
sources DOAJ
author Adilson Guilherme
David J. Pedersen
Felipe Henriques
Alexander H. Bedard
Elizabeth Henchey
Mark Kelly
Donald A. Morgan
Kamal Rahmouni
Michael P. Czech
spellingShingle Adilson Guilherme
David J. Pedersen
Felipe Henriques
Alexander H. Bedard
Elizabeth Henchey
Mark Kelly
Donald A. Morgan
Kamal Rahmouni
Michael P. Czech
Neuronal modulation of brown adipose activity through perturbation of white adipocyte lipogenesis
Molecular Metabolism
author_facet Adilson Guilherme
David J. Pedersen
Felipe Henriques
Alexander H. Bedard
Elizabeth Henchey
Mark Kelly
Donald A. Morgan
Kamal Rahmouni
Michael P. Czech
author_sort Adilson Guilherme
title Neuronal modulation of brown adipose activity through perturbation of white adipocyte lipogenesis
title_short Neuronal modulation of brown adipose activity through perturbation of white adipocyte lipogenesis
title_full Neuronal modulation of brown adipose activity through perturbation of white adipocyte lipogenesis
title_fullStr Neuronal modulation of brown adipose activity through perturbation of white adipocyte lipogenesis
title_full_unstemmed Neuronal modulation of brown adipose activity through perturbation of white adipocyte lipogenesis
title_sort neuronal modulation of brown adipose activity through perturbation of white adipocyte lipogenesis
publisher Elsevier
series Molecular Metabolism
issn 2212-8778
publishDate 2018-10-01
description Objective: Crosstalk between adipocytes and local neurons may be an important regulatory mechanism to control energy homeostasis. We previously reported that perturbation of adipocyte de novo lipogenesis (DNL) by deletion of fatty acid synthase (FASN) expands sympathetic neurons within white adipose tissue (WAT) and stimulates the appearance of “beige” adipocytes. Here we tested whether WAT DNL activity can also influence neuronal regulation and thermogenesis in brown adipose tissue (BAT). Methods and results: Induced deletion of FASN in all adipocytes in mature mice (iAdFASNKO) enhanced sympathetic innervation and neuronal activity as well as UCP1 expression in both WAT and BAT. This increased sympathetic innervation could be observed at both 22 °C and 30 °C, indicating it is not a response to heat loss but rather adipocyte signaling. In contrast, selective ablation of FASN in brown adipocytes of mice (iUCP1FASNKO) failed to modulate sympathetic innervation and the thermogenic program in BAT. Surprisingly, DNL in brown adipocytes was also dispensable in maintaining euthermia when UCP1FASNKO mice were cold-exposed. Conclusion: These results indicate that DNL in white adipocytes influences long distance signaling to BAT, which can modify BAT sympathetic innervation and expression of genes involved in thermogenesis. Keywords: Adipocytes, Lipogenesis, Brown adipose tissue, Thermogenesis, Sensory nerve, Sympathetic nerve, SNS outflow
url http://www.sciencedirect.com/science/article/pii/S2212877818305180
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