Molecular characterization of vancomycin-resistant enterococci isolated from a hospital in Beijing, China

Objective: To investigate the occurrence of vancomycin-resistant enterococci (VRE) isolated from patients in Peking Union Medical College Hospital, Beijing, China from 2011 to 2017, and to evaluate their resistance mechanisms and genetic relatedness. Methods: All isolates were identified using matri...

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Main Authors: Hong-Li Sun, Chang Liu, Jing-Jia Zhang, Yu-Ming Zhou, Ying-Chun Xu
Format: Article
Language:English
Published: Elsevier 2019-06-01
Series:Journal of Microbiology, Immunology and Infection
Online Access:http://www.sciencedirect.com/science/article/pii/S1684118218305322
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spelling doaj-158a3faee4744123b1135f50781bc9742020-11-25T01:05:18ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822019-06-01523433442Molecular characterization of vancomycin-resistant enterococci isolated from a hospital in Beijing, ChinaHong-Li Sun0Chang Liu1Jing-Jia Zhang2Yu-Ming Zhou3Ying-Chun Xu4Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, ChinaBeijing Huaxin Hospital, The First Hospital of Tsinghua University, Beijing, ChinaPeking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, ChinaPeking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, ChinaPeking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China; Corresponding author. Department of Clinical Laboratory, Peking Union Medical College Hospital, No 1, Shuai-Fu-Yuan, Dong-Cheng District, Beijing, China.Objective: To investigate the occurrence of vancomycin-resistant enterococci (VRE) isolated from patients in Peking Union Medical College Hospital, Beijing, China from 2011 to 2017, and to evaluate their resistance mechanisms and genetic relatedness. Methods: All isolates were identified using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF). Antibiotic susceptibility testing was performed using the broth microdilution method. Molecular characterization were detected by PCR and sequencing. Genotyping of VRE isolates was performed by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) analysis. Virulence genes were detected by multiplex PCR. Results: A total of 87 consecutive VRE were collected, including 84 isolates of vancomycin resistant Enterococcus faecium (VREfm) and 3 isolates of Enterococcus faecalis (VREfs). Urine (40.2%, 35/87) and blood (17.2%, 15/87) were the most commonly specimens. All VREfm isolates were resistant to ampicillin, and were susceptible to daptomycin, linezolid and tigecycline. The resistant rate of teicoplanin was 47.6%. All of the VREfm isolates carried the vanA gene, no isolates carried vanB. 11.9% (10/84) VREfm isolates carried both vanA and vanM. Among them, 76.2% (64/84) and 66.7% (56/84) carried esp and hyl, respectively. The 3 vancomycin resistant E. faecalis (VREfs) isolates were varied, and only one carried vanB. A total of 3 and 18 STs were detected among VREfs and VREfm strains, respectively. PFGE results indicated a genetic diversity among VREfm isolates. Conclusion: This study confirms that VREfm isolates associated with ST78 were the main epidemic lineage responsible for nosocomial infections in China, as were also observed in other nations worldwide. Keywords: Enterococcus, Antimicrobial susceptibility testing, Van gene, Virulence genes, Molecular typinghttp://www.sciencedirect.com/science/article/pii/S1684118218305322
collection DOAJ
language English
format Article
sources DOAJ
author Hong-Li Sun
Chang Liu
Jing-Jia Zhang
Yu-Ming Zhou
Ying-Chun Xu
spellingShingle Hong-Li Sun
Chang Liu
Jing-Jia Zhang
Yu-Ming Zhou
Ying-Chun Xu
Molecular characterization of vancomycin-resistant enterococci isolated from a hospital in Beijing, China
Journal of Microbiology, Immunology and Infection
author_facet Hong-Li Sun
Chang Liu
Jing-Jia Zhang
Yu-Ming Zhou
Ying-Chun Xu
author_sort Hong-Li Sun
title Molecular characterization of vancomycin-resistant enterococci isolated from a hospital in Beijing, China
title_short Molecular characterization of vancomycin-resistant enterococci isolated from a hospital in Beijing, China
title_full Molecular characterization of vancomycin-resistant enterococci isolated from a hospital in Beijing, China
title_fullStr Molecular characterization of vancomycin-resistant enterococci isolated from a hospital in Beijing, China
title_full_unstemmed Molecular characterization of vancomycin-resistant enterococci isolated from a hospital in Beijing, China
title_sort molecular characterization of vancomycin-resistant enterococci isolated from a hospital in beijing, china
publisher Elsevier
series Journal of Microbiology, Immunology and Infection
issn 1684-1182
publishDate 2019-06-01
description Objective: To investigate the occurrence of vancomycin-resistant enterococci (VRE) isolated from patients in Peking Union Medical College Hospital, Beijing, China from 2011 to 2017, and to evaluate their resistance mechanisms and genetic relatedness. Methods: All isolates were identified using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF). Antibiotic susceptibility testing was performed using the broth microdilution method. Molecular characterization were detected by PCR and sequencing. Genotyping of VRE isolates was performed by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) analysis. Virulence genes were detected by multiplex PCR. Results: A total of 87 consecutive VRE were collected, including 84 isolates of vancomycin resistant Enterococcus faecium (VREfm) and 3 isolates of Enterococcus faecalis (VREfs). Urine (40.2%, 35/87) and blood (17.2%, 15/87) were the most commonly specimens. All VREfm isolates were resistant to ampicillin, and were susceptible to daptomycin, linezolid and tigecycline. The resistant rate of teicoplanin was 47.6%. All of the VREfm isolates carried the vanA gene, no isolates carried vanB. 11.9% (10/84) VREfm isolates carried both vanA and vanM. Among them, 76.2% (64/84) and 66.7% (56/84) carried esp and hyl, respectively. The 3 vancomycin resistant E. faecalis (VREfs) isolates were varied, and only one carried vanB. A total of 3 and 18 STs were detected among VREfs and VREfm strains, respectively. PFGE results indicated a genetic diversity among VREfm isolates. Conclusion: This study confirms that VREfm isolates associated with ST78 were the main epidemic lineage responsible for nosocomial infections in China, as were also observed in other nations worldwide. Keywords: Enterococcus, Antimicrobial susceptibility testing, Van gene, Virulence genes, Molecular typing
url http://www.sciencedirect.com/science/article/pii/S1684118218305322
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