Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver
We aimed in the present work is to study the effects of fosetyl-Al and fenamidone based fungicide (“Verita WG”) on glycogen storage and expression of lipid droplets in common carp (Cyprinus carpio, L.) liver. Concentrations of the test chemical were 30 mg/L, 38 mg/L and 50 mg/L under laboratory co...
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Union of Scientists in Bulgaria
2013-12-01
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doaj-1584bf27ab634769a13142438de7718f2021-02-02T07:26:56ZengUnion of Scientists in BulgariaEcologia Balkanica1314-02131313-99402013-12-01529197Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) LiverElenka Georgieva0Pepa Atanasova1Iliana Velcheva2Stela Stoyanova3Vesela Yancheva4Department of Developmental Biology, Faculty of Biology, Plovdiv University, 4000 Plovdiv, BULGARIADepartment of Anatomy, Histology and Embryology, Medical University of Plovdiv, 4000 Plovdiv, BULGARIADepartment of Ecology and Environmental Conservation, Faculty of Biology, Plovdiv University, 4000 Plovdiv, BULGARIADepartment of Ecology and Environmental Conservation, Faculty of Biology, Plovdiv University, 4000 Plovdiv, BULGARIADepartment of Ecology and Environmental Conservation, Faculty of Biology, Plovdiv University, 4000 Plovdiv, BULGARIAWe aimed in the present work is to study the effects of fosetyl-Al and fenamidone based fungicide (“Verita WG”) on glycogen storage and expression of lipid droplets in common carp (Cyprinus carpio, L.) liver. Concentrations of the test chemical were 30 mg/L, 38 mg/L and 50 mg/L under laboratory conditions. We used PAS-reaction for detection of glycogen storage and Sudan III staining for detection of lipid droplets in common carp hepatocytes. Hence, we found that the amount of glycogen and the fat storage in the liver increased proportionally with the increased fungicide concentrations. We also found conglomerates of accumulated glycogen in certain hepatocytes at all used concentrations. Overall, the results demonstrated enhanced glyconeogenesis and fat accumulation in the common carp liver, exposed to the test chemical. http://web.uni-plovdiv.bg/mollov/EB/2013_vol5_iss2/%5b91-97%5d_eb.13125.pdfpesticideshistopathologyPAS-reactionfatty degenerationlivercommon carp |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elenka Georgieva Pepa Atanasova Iliana Velcheva Stela Stoyanova Vesela Yancheva |
spellingShingle |
Elenka Georgieva Pepa Atanasova Iliana Velcheva Stela Stoyanova Vesela Yancheva Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver Ecologia Balkanica pesticides histopathology PAS-reaction fatty degeneration liver common carp |
author_facet |
Elenka Georgieva Pepa Atanasova Iliana Velcheva Stela Stoyanova Vesela Yancheva |
author_sort |
Elenka Georgieva |
title |
Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver |
title_short |
Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver |
title_full |
Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver |
title_fullStr |
Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver |
title_full_unstemmed |
Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver |
title_sort |
histochemical effects of “verita wg” on glycogen and lipid storage in common carp (cyprinus carpio l.) liver |
publisher |
Union of Scientists in Bulgaria |
series |
Ecologia Balkanica |
issn |
1314-0213 1313-9940 |
publishDate |
2013-12-01 |
description |
We aimed in the present work is to study the effects of fosetyl-Al and fenamidone based fungicide (“Verita WG”) on glycogen storage and expression of lipid droplets in common carp
(Cyprinus carpio, L.) liver. Concentrations of the test chemical were 30 mg/L, 38 mg/L and 50 mg/L under laboratory conditions. We used PAS-reaction for detection of glycogen storage and Sudan III staining for detection of lipid droplets in common carp hepatocytes. Hence, we found that the amount of glycogen and the fat storage in the liver increased proportionally with the increased fungicide concentrations. We also found conglomerates of accumulated glycogen in certain hepatocytes at all used concentrations. Overall, the results demonstrated enhanced glyconeogenesis and fat accumulation in the common carp liver, exposed to the test chemical.
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topic |
pesticides histopathology PAS-reaction fatty degeneration liver common carp |
url |
http://web.uni-plovdiv.bg/mollov/EB/2013_vol5_iss2/%5b91-97%5d_eb.13125.pdf |
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