Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver

We aimed in the present work is to study the effects of fosetyl-Al and fenamidone based fungicide (“Verita WG”) on glycogen storage and expression of lipid droplets in common carp (Cyprinus carpio, L.) liver. Concentrations of the test chemical were 30 mg/L, 38 mg/L and 50 mg/L under laboratory co...

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Main Authors: Elenka Georgieva, Pepa Atanasova, Iliana Velcheva, Stela Stoyanova, Vesela Yancheva
Format: Article
Language:English
Published: Union of Scientists in Bulgaria 2013-12-01
Series:Ecologia Balkanica
Subjects:
Online Access:http://web.uni-plovdiv.bg/mollov/EB/2013_vol5_iss2/%5b91-97%5d_eb.13125.pdf
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spelling doaj-1584bf27ab634769a13142438de7718f2021-02-02T07:26:56ZengUnion of Scientists in BulgariaEcologia Balkanica1314-02131313-99402013-12-01529197Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) LiverElenka Georgieva0Pepa Atanasova1Iliana Velcheva2Stela Stoyanova3Vesela Yancheva4Department of Developmental Biology, Faculty of Biology, Plovdiv University, 4000 Plovdiv, BULGARIADepartment of Anatomy, Histology and Embryology, Medical University of Plovdiv, 4000 Plovdiv, BULGARIADepartment of Ecology and Environmental Conservation, Faculty of Biology, Plovdiv University, 4000 Plovdiv, BULGARIADepartment of Ecology and Environmental Conservation, Faculty of Biology, Plovdiv University, 4000 Plovdiv, BULGARIADepartment of Ecology and Environmental Conservation, Faculty of Biology, Plovdiv University, 4000 Plovdiv, BULGARIAWe aimed in the present work is to study the effects of fosetyl-Al and fenamidone based fungicide (“Verita WG”) on glycogen storage and expression of lipid droplets in common carp (Cyprinus carpio, L.) liver. Concentrations of the test chemical were 30 mg/L, 38 mg/L and 50 mg/L under laboratory conditions. We used PAS-reaction for detection of glycogen storage and Sudan III staining for detection of lipid droplets in common carp hepatocytes. Hence, we found that the amount of glycogen and the fat storage in the liver increased proportionally with the increased fungicide concentrations. We also found conglomerates of accumulated glycogen in certain hepatocytes at all used concentrations. Overall, the results demonstrated enhanced glyconeogenesis and fat accumulation in the common carp liver, exposed to the test chemical. http://web.uni-plovdiv.bg/mollov/EB/2013_vol5_iss2/%5b91-97%5d_eb.13125.pdfpesticideshistopathologyPAS-reactionfatty degenerationlivercommon carp
collection DOAJ
language English
format Article
sources DOAJ
author Elenka Georgieva
Pepa Atanasova
Iliana Velcheva
Stela Stoyanova
Vesela Yancheva
spellingShingle Elenka Georgieva
Pepa Atanasova
Iliana Velcheva
Stela Stoyanova
Vesela Yancheva
Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver
Ecologia Balkanica
pesticides
histopathology
PAS-reaction
fatty degeneration
liver
common carp
author_facet Elenka Georgieva
Pepa Atanasova
Iliana Velcheva
Stela Stoyanova
Vesela Yancheva
author_sort Elenka Georgieva
title Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver
title_short Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver
title_full Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver
title_fullStr Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver
title_full_unstemmed Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L.) Liver
title_sort histochemical effects of “verita wg” on glycogen and lipid storage in common carp (cyprinus carpio l.) liver
publisher Union of Scientists in Bulgaria
series Ecologia Balkanica
issn 1314-0213
1313-9940
publishDate 2013-12-01
description We aimed in the present work is to study the effects of fosetyl-Al and fenamidone based fungicide (“Verita WG”) on glycogen storage and expression of lipid droplets in common carp (Cyprinus carpio, L.) liver. Concentrations of the test chemical were 30 mg/L, 38 mg/L and 50 mg/L under laboratory conditions. We used PAS-reaction for detection of glycogen storage and Sudan III staining for detection of lipid droplets in common carp hepatocytes. Hence, we found that the amount of glycogen and the fat storage in the liver increased proportionally with the increased fungicide concentrations. We also found conglomerates of accumulated glycogen in certain hepatocytes at all used concentrations. Overall, the results demonstrated enhanced glyconeogenesis and fat accumulation in the common carp liver, exposed to the test chemical.
topic pesticides
histopathology
PAS-reaction
fatty degeneration
liver
common carp
url http://web.uni-plovdiv.bg/mollov/EB/2013_vol5_iss2/%5b91-97%5d_eb.13125.pdf
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