Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene

Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene

The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele). The ICM was isolated us...

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Main Authors: Cristian Miere, Heema Hewitson, Liani Devito, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506116000143
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spelling doaj-1561c21ef2204fe4a72da64404e07c5b2020-11-24T21:50:06ZengElsevierStem Cell Research1873-50611876-77532016-03-0116234234410.1016/j.scr.2016.01.004Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK geneCristian MiereHeema HewitsonLiani DevitoVictoria WoodNeli KadevaGlenda CornwellStefano CodognottoEmma StephensonDusko IlicThe KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.http://www.sciencedirect.com/science/article/pii/S1873506116000143
collection DOAJ
language English
format Article
sources DOAJ
author Cristian Miere
Heema Hewitson
Liani Devito
Victoria Wood
Neli Kadeva
Glenda Cornwell
Stefano Codognotto
Emma Stephenson
Dusko Ilic
spellingShingle Cristian Miere
Heema Hewitson
Liani Devito
Victoria Wood
Neli Kadeva
Glenda Cornwell
Stefano Codognotto
Emma Stephenson
Dusko Ilic
Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene
Stem Cell Research
author_facet Cristian Miere
Heema Hewitson
Liani Devito
Victoria Wood
Neli Kadeva
Glenda Cornwell
Stefano Codognotto
Emma Stephenson
Dusko Ilic
author_sort Cristian Miere
title Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene
title_short Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene
title_full Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene
title_fullStr Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene
title_full_unstemmed Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene
title_sort generation of kcl018 research grade human embryonic stem cell line carrying a mutation in the dmpk gene
publisher Elsevier
series Stem Cell Research
issn 1873-5061
1876-7753
publishDate 2016-03-01
description The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.
url http://www.sciencedirect.com/science/article/pii/S1873506116000143
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