Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation

Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation

Abstract Background The pollen tube (PT) serves as a model system for investigating plant cell growth and morphogenesis. Ultrastructural studies are indispensable to complement data from physiological and genetic analyses, yet an effective method is lacking for PTs of the model plant Arabidopsis tha...

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Bibliographic Details
Main Authors: Tohnyui Ndinyanka Fabrice, Andres Kaech, Gery Barmettler, Christof Eichenberger, J. Paul Knox, Ueli Grossniklaus, Christoph Ringli
Format: Article
Language:English
Published: BMC 2017-10-01
Series:BMC Plant Biology
Subjects:
Arabidopsis
Callose
Cell wall
Electron microscopy
Immunolabeling
Pollen
Online Access:http://link.springer.com/article/10.1186/s12870-017-1136-x

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http://link.springer.com/article/10.1186/s12870-017-1136-x

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