Flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.

Flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.

An optimal technology for cell cycle analysis would allow the concomitant measurement of apoptosis, G0, G1, S, G2 and M phases in combination with cell surface phenotyping. We have developed an easy method in flow cytometry allowing this discrimination in an only two-color fluorescent plot. It is ba...

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Bibliographic Details
Main Authors: Christine Vignon, Christelle Debeissat, Marie-Thérèse Georget, Didier Bouscary, Emmanuel Gyan, Philippe Rosset, Olivier Herault
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23935867/?tool=EBI
Description
Summary:An optimal technology for cell cycle analysis would allow the concomitant measurement of apoptosis, G0, G1, S, G2 and M phases in combination with cell surface phenotyping. We have developed an easy method in flow cytometry allowing this discrimination in an only two-color fluorescent plot. It is based on the concomitant use of 7-amino-actinomycin D and the antibodies anti-Ki67 and anti-phospho(Ser10)-histone H3, both conjugated to Alexa Fluor®488 to discriminate G0 and M phases, respectively. The method is particularly valuable in a clinical setting as verified in our laboratory by analyzing human leukemic cells from marrow samples or after exposure to cell cycle modifiers.
ISSN:1932-6203

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