In Vitro Characterization of the Two-Stage Non-Classical Reassembly Pathway of S-Layers

In Vitro Characterization of the Two-Stage Non-Classical Reassembly Pathway of S-Layers

The recombinant bacterial surface layer (S-layer) protein rSbpA of Lysinibacillus sphaericus CCM 2177 is an ideal model system to study non-classical nucleation and growth of protein crystals at surfaces since the recrystallization process may be separated into two distinct steps: (i) adsorption of...

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Bibliographic Details
Main Authors: Andreas Breitwieser, Jagoba Iturri, Jose-Luis Toca-Herrera, Uwe B. Sleytr, Dietmar Pum
Format: Article
Language:English
Published: MDPI AG 2017-02-01
Series:International Journal of Molecular Sciences
Subjects:
S-layer proteins
two-step crystallization
self-assembly kinetics
non-classical crystal growth
Ca2+ binding
Online Access:http://www.mdpi.com/1422-0067/18/2/400

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