Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin
The innate immune response plays an important role in the pathophysiology of acute respiratory distress syndrome (ARDS). Glutamine (Gln) decreases lung inflammation in experimental ARDS, but its impact on the formation of extracellular traps (ETs) in the lung is unknown. In a mouse model of endotoxi...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-04-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/11/4/831 |
| id |
doaj-150ed4201488499fa1617573af0421c0 |
|---|---|
| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Gisele Pena de Oliveira Jamil Zola Kitoko Phillipe de Souza Lima-Gomes Natália Cadaxo Rochael Carla Cristina de Araújo Pâmella Nowaski Lugon Heloísa Lopes dos Santos Eduarda Gabrielle Lopes Martins Felipe Mateus Ornellas Helena D’Anunciação de Oliveira Marcelo Marcos Morales Priscilla Christina Olsen Antônio Galina Pedro Leme Silva Elvira Maria Saraiva Paolo Pelosi Patricia Rieken Macedo Rocco |
| spellingShingle |
Gisele Pena de Oliveira Jamil Zola Kitoko Phillipe de Souza Lima-Gomes Natália Cadaxo Rochael Carla Cristina de Araújo Pâmella Nowaski Lugon Heloísa Lopes dos Santos Eduarda Gabrielle Lopes Martins Felipe Mateus Ornellas Helena D’Anunciação de Oliveira Marcelo Marcos Morales Priscilla Christina Olsen Antônio Galina Pedro Leme Silva Elvira Maria Saraiva Paolo Pelosi Patricia Rieken Macedo Rocco Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin Nutrients glutamine pulmonary acute respiratory distress syndrome lung mechanics extracellular traps reactive oxygen species |
| author_facet |
Gisele Pena de Oliveira Jamil Zola Kitoko Phillipe de Souza Lima-Gomes Natália Cadaxo Rochael Carla Cristina de Araújo Pâmella Nowaski Lugon Heloísa Lopes dos Santos Eduarda Gabrielle Lopes Martins Felipe Mateus Ornellas Helena D’Anunciação de Oliveira Marcelo Marcos Morales Priscilla Christina Olsen Antônio Galina Pedro Leme Silva Elvira Maria Saraiva Paolo Pelosi Patricia Rieken Macedo Rocco |
| author_sort |
Gisele Pena de Oliveira |
| title |
Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin |
| title_short |
Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin |
| title_full |
Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin |
| title_fullStr |
Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin |
| title_full_unstemmed |
Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin |
| title_sort |
glutamine therapy reduces inflammation and extracellular trap release in experimental acute respiratory distress syndrome of pulmonary origin |
| publisher |
MDPI AG |
| series |
Nutrients |
| issn |
2072-6643 |
| publishDate |
2019-04-01 |
| description |
The innate immune response plays an important role in the pathophysiology of acute respiratory distress syndrome (ARDS). Glutamine (Gln) decreases lung inflammation in experimental ARDS, but its impact on the formation of extracellular traps (ETs) in the lung is unknown. In a mouse model of endotoxin-induced pulmonary ARDS, the effects of Gln treatment on leukocyte counts and ET content in bronchoalveolar lavage fluid (BALF), inflammatory profile in lung tissue, and lung morphofunction were evaluated in vivo. Furthermore, ET formation, reactive oxygen species (ROS) production, glutathione peroxidase (GPx), and glutathione reductase (GR) activities were tested in vitro. Our in vivo results demonstrated that Gln treatment reduced ET release (as indicated by cell-free-DNA content and myeloperoxidase activity), decreased lung inflammation (reductions in interferon-γ and increases in interleukin-10 levels), and improved lung morpho-function (decreased static lung elastance and alveolar collapse) in comparison with ARDS animals treated with saline. Moreover, Gln reduced ET and ROS formation in BALF cells stimulated with lipopolysaccharide in vitro, but it did not alter GPx or GR activity. In this model of endotoxin-induced pulmonary ARDS, treatment with Gln reduced pulmonary functional and morphological impairment, inflammation, and ET release in the lung. |
| topic |
glutamine pulmonary acute respiratory distress syndrome lung mechanics extracellular traps reactive oxygen species |
| url |
https://www.mdpi.com/2072-6643/11/4/831 |
| work_keys_str_mv |
AT giselepenadeoliveira glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT jamilzolakitoko glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT phillipedesouzalimagomes glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT nataliacadaxorochael glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT carlacristinadearaujo glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT pamellanowaskilugon glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT heloisalopesdossantos glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT eduardagabriellelopesmartins glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT felipemateusornellas glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT helenadanunciacaodeoliveira glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT marcelomarcosmorales glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT priscillachristinaolsen glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT antoniogalina glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT pedrolemesilva glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT elviramariasaraiva glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT paolopelosi glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT patriciariekenmacedorocco glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin |
| _version_ |
1725792922123632640 |
| spelling |
doaj-150ed4201488499fa1617573af0421c02020-11-24T22:15:48ZengMDPI AGNutrients2072-66432019-04-0111483110.3390/nu11040831nu11040831Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary OriginGisele Pena de Oliveira0Jamil Zola Kitoko1Phillipe de Souza Lima-Gomes2Natália Cadaxo Rochael3Carla Cristina de Araújo4Pâmella Nowaski Lugon5Heloísa Lopes dos Santos6Eduarda Gabrielle Lopes Martins7Felipe Mateus Ornellas8Helena D’Anunciação de Oliveira9Marcelo Marcos Morales10Priscilla Christina Olsen11Antônio Galina12Pedro Leme Silva13Elvira Maria Saraiva14Paolo Pelosi15Patricia Rieken Macedo Rocco16Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Leishmaniasis Immunobiology, Immunology Department, Paulo de Góes Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Leishmaniasis Immunobiology, Immunology Department, Paulo de Góes Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Bioenergetics and Mitochondrial Physiology, Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Clinical Bacteriology and Immunology, Department of Toxicological and Clinical Analysis, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Bioenergetics and Mitochondrial Physiology, Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratory of Leishmaniasis Immunobiology, Immunology Department, Paulo de Góes Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilDepartment of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, 16132 Genoa, ItalyLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilThe innate immune response plays an important role in the pathophysiology of acute respiratory distress syndrome (ARDS). Glutamine (Gln) decreases lung inflammation in experimental ARDS, but its impact on the formation of extracellular traps (ETs) in the lung is unknown. In a mouse model of endotoxin-induced pulmonary ARDS, the effects of Gln treatment on leukocyte counts and ET content in bronchoalveolar lavage fluid (BALF), inflammatory profile in lung tissue, and lung morphofunction were evaluated in vivo. Furthermore, ET formation, reactive oxygen species (ROS) production, glutathione peroxidase (GPx), and glutathione reductase (GR) activities were tested in vitro. Our in vivo results demonstrated that Gln treatment reduced ET release (as indicated by cell-free-DNA content and myeloperoxidase activity), decreased lung inflammation (reductions in interferon-γ and increases in interleukin-10 levels), and improved lung morpho-function (decreased static lung elastance and alveolar collapse) in comparison with ARDS animals treated with saline. Moreover, Gln reduced ET and ROS formation in BALF cells stimulated with lipopolysaccharide in vitro, but it did not alter GPx or GR activity. In this model of endotoxin-induced pulmonary ARDS, treatment with Gln reduced pulmonary functional and morphological impairment, inflammation, and ET release in the lung.https://www.mdpi.com/2072-6643/11/4/831glutaminepulmonary acute respiratory distress syndromelung mechanicsextracellular trapsreactive oxygen species |