Collagen Extracted from Persian Gulf Squid Exhibits Anti-Cytotoxic Properties on Apple Pectic Treated Cells: Assessment in an In Vitro Bioassay Model

• Main Authors: Ladan DELPHI, Houri SEPEHRI, Elahe MOTEVASELI, Mohammad Reza KHORRAMIZADEH
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2016-10-01
• Series: Iranian Journal of Public Health
• Online Access: http://ijph.tums.ac.ir/index.php/ijph/article/view/7607
• ISSN: 2251-6085; 2251-6093

<p><strong>Background:</strong> Collagen-based three-dimensional (3D) in vitro systems have been introduced to study the physiological states of cells. As a biomolecule, collagen is usually extracted from terrestrial animals whilst aquatic animals like squid contain large amounts of collagen.</p><p><strong>Methods:</strong> In order to make effective use of marine organisms, we selected Persian Gulf squid in 2015 to extract the required collagen. Then, a 3D culture system based on the extracted collagen was applied to investigate cellular mechanisms in a native microenvironment. The formed collagen gel was used to investigate the growth of MDA-MB-231 breast cancer cells as well as responses to pectic acid.</p><p><strong>Results:</strong> The results revealed that the extracted collagen contained α, ß and γ components with high water holding capacity. This collagen formed a gel-like structure, which could promote the proliferation of MDA-MB-231 breast cancer cells. The MDA-MB-231 cells’ viability in presence of pectic acid, demonstrating the cells’ behavior in a 3D culture system<strong>. </strong></p><p><strong>Conclusion:</strong> It seems that the collagen extracted from squid skin has type I collagen properties. It might be used as a substrate in 3D cell culture systems.</p><p><strong> </strong></p><p><strong>Keywords:</strong><strong> </strong>Cell culture, Collagen, Pectic acid, Squid, 3D culture system</p><p><strong> </strong></p>