The enzyme-linked immunosorbent assay (ELISA): history, theory and application

The enzyme-linked immunosorbent assay (ELISA): history, theory and application

Enzyme-linked immunosorbent assay (ELISA) play a significant role in the development of many important fields of biology and medicine, including the detection of the dangerous infections agents. In the present review there is described the history of ELISA’s invention, further improvement of this me...

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Main Authors: Yu. N. Tarakanova, A. D. Dmitriev, D. A. Dmitriev, V. F. Lavrov, Yu. S. Massino, А. A. Pechelyulko, O. L. Segal
Format: Article
Language:Russian
Published: Central Research Institute for Epidemiology 2019-08-01
Series: Журнал микробиологии, эпидемиологии и иммунобиологии
Subjects:
enzyme-linked immunosorbent assays
analytical sensitivity
monoclonal antibodies
affinity
avidity
sandwich elisa
Online Access:https://microbiol.elpub.ru/jour/article/view/376
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spelling doaj-1498dbf57ac34855838b168a52cab4262021-07-28T13:07:52ZrusCentral Research Institute for Epidemiology Журнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132019-08-010311712510.36233/0372-9311-2019-3-117-125368The enzyme-linked immunosorbent assay (ELISA): history, theory and applicationYu. N. Tarakanova0A. D. Dmitriev1D. A. Dmitriev2V. F. Lavrov3Yu. S. Massino4А. A. Pechelyulko5O. L. Segal6Mechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and Sera; Russian Medical Academy of Continuous Professional EducationMechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraEnzyme-linked immunosorbent assay (ELISA) play a significant role in the development of many important fields of biology and medicine, including the detection of the dangerous infections agents. In the present review there is described the history of ELISA’s invention, further improvement of this method and application in modern biology and medicine. The special accent is made on factors influencing antibody-antigen interactions on the solid phase and their use to increase the analytical sensitivity of the method.https://microbiol.elpub.ru/jour/article/view/376enzyme-linked immunosorbent assaysanalytical sensitivitymonoclonal antibodiesaffinityaviditysandwich elisa
collection DOAJ
language Russian
format Article
sources DOAJ
author Yu. N. Tarakanova
A. D. Dmitriev
D. A. Dmitriev
V. F. Lavrov
Yu. S. Massino
А. A. Pechelyulko
O. L. Segal
spellingShingle Yu. N. Tarakanova
A. D. Dmitriev
D. A. Dmitriev
V. F. Lavrov
Yu. S. Massino
А. A. Pechelyulko
O. L. Segal
The enzyme-linked immunosorbent assay (ELISA): history, theory and application
Журнал микробиологии, эпидемиологии и иммунобиологии
enzyme-linked immunosorbent assays
analytical sensitivity
monoclonal antibodies
affinity
avidity
sandwich elisa
author_facet Yu. N. Tarakanova
A. D. Dmitriev
D. A. Dmitriev
V. F. Lavrov
Yu. S. Massino
А. A. Pechelyulko
O. L. Segal
author_sort Yu. N. Tarakanova
title The enzyme-linked immunosorbent assay (ELISA): history, theory and application
title_short The enzyme-linked immunosorbent assay (ELISA): history, theory and application
title_full The enzyme-linked immunosorbent assay (ELISA): history, theory and application
title_fullStr The enzyme-linked immunosorbent assay (ELISA): history, theory and application
title_full_unstemmed The enzyme-linked immunosorbent assay (ELISA): history, theory and application
title_sort enzyme-linked immunosorbent assay (elisa): history, theory and application
publisher Central Research Institute for Epidemiology
series Журнал микробиологии, эпидемиологии и иммунобиологии
issn 0372-9311
2686-7613
publishDate 2019-08-01
description Enzyme-linked immunosorbent assay (ELISA) play a significant role in the development of many important fields of biology and medicine, including the detection of the dangerous infections agents. In the present review there is described the history of ELISA’s invention, further improvement of this method and application in modern biology and medicine. The special accent is made on factors influencing antibody-antigen interactions on the solid phase and their use to increase the analytical sensitivity of the method.
topic enzyme-linked immunosorbent assays
analytical sensitivity
monoclonal antibodies
affinity
avidity
sandwich elisa
url https://microbiol.elpub.ru/jour/article/view/376
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