Off-Target Analysis in Gene Editing and Applications for Clinical Translation of CRISPR/Cas9 in HIV-1 Therapy

Off-Target Analysis in Gene Editing and Applications for Clinical Translation of CRISPR/Cas9 in HIV-1 Therapy

As genome-editing nucleases move toward broader clinical applications, the need to define the limits of their specificity and efficiency increases. A variety of approaches for nuclease cleavage detection have been developed, allowing a full-genome survey of the targeting landscape and the detection...

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Bibliographic Details
Main Authors: Andrew Atkins, Cheng-Han Chung, Alexander G. Allen, Will Dampier, Theodore E. Gurrola, Ilker K. Sariyer, Michael R. Nonnemacher, Brian Wigdahl
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-08-01
Series:Frontiers in Genome Editing
Subjects:
HIV-1
off-target
CRISPR
unbiased genome-wide
GUIDE-seq
CIRCLE-seq
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2021.673022/full

Internet

https://www.frontiersin.org/articles/10.3389/fgeed.2021.673022/full

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