Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics

Introduction. Wild-crafting leads to the local extinction of many medicinal plants that are rich in phenolic substances. In vitro cultivation of cells and organs of higher plants can be the optimal solution to this problem. The research objective was to study the biosynthetic activity of in vitro ex...

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Main Authors: Irina S. Milentyeva, Violeta M. Le, Oksana V. Kozlova, Natalia S. Velichkovich, Anastasiya M. Fedorova, Anna I. Loseva, Vladimir P. Yustratov
Format: Article
Language:English
Published: Kemerovo State University 2021-04-01
Series:Foods and Raw Materials
Subjects:
Online Access:http://jfrm.ru/files/archive/17/Milentyeva.pdf
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spelling doaj-14722654a2ba47c58c38d655375252f52021-05-19T08:22:19ZengKemerovo State UniversityFoods and Raw Materials2308-40572310-95992021-04-019115316310.21603/2308-4057-2021-1-153-163Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristicsIrina S. Milentyeva0https://orcid.org/0000-0002-3536-562XVioleta M. Le1https://orcid.org/0000-0002-9546-6633Oksana V. Kozlova2https://orcid.org/0000-0002-2960-0216Natalia S. Velichkovich3https://orcid.org/0000-0002-9061-1256Anastasiya M. Fedorova4https://orcid.org/0000-0002-8071-4411Anna I. Loseva5https://orcid.org/0000-0003-4037-2653Vladimir P. Yustratov6Kemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaIntroduction. Wild-crafting leads to the local extinction of many medicinal plants that are rich in phenolic substances. In vitro cultivation of cells and organs of higher plants can be the optimal solution to this problem. The research objective was to study the biosynthetic activity of in vitro extracts of wild Siberian plants. Study objects and methods. The study featured callus, cell suspension, and hairy root extracts of such Siberian medicinal plants as Eleutherococcus senticosus, Codonopsis pilosula, Platanthera bifolia, and Saposhnikovia divaricata. They were obtained by in vitro cultivation using modified nutrient media of Murashige and Skoog and Gamborg. The content of secondary metabolites was studied using the methods of thin-layer and high-performance liquid chromatography. A set of in vitro experiments tested the antioxidant and antimicrobial activity of the extracts. Results and discussion. All the samples demonstrated a high content of secondary metabolites of phenolic nature. Flavonoglycosides, apigenin, and rutin were found to be the predominant biologically active substances in the callus extracts. Flavonoglycosides dominated in the suspension extracts. The root extracts contained more caffeic acid, rutin, ecdysteroids, quercetin, apigenin, cardiofolin, and coleofolide than the callus and suspension cultures. The list of prevailing secondary metabolites in the root extracts included rutin, apigenin, coleofolide, and quercetin. All the extracts showed antimicrobial and antioxidant activity. Conclusion. All the extracts demonstrated good antioxidant and antimicrobial properties. Therefore, they can be used for the production of pharmaceuticals and biologically active food supplements as they can be helpful against infectious diseases, as well as oncological, cardiovascular, and neurodegenerative diseases linked to oxidative stress.http://jfrm.ru/files/archive/17/Milentyeva.pdfcallus culturecell suspension culturehairy rootsmedicinal plantsecondary metabolitephenolic substancesantioxidantoxidative stressantimicrobial propertiesextraction
collection DOAJ
language English
format Article
sources DOAJ
author Irina S. Milentyeva
Violeta M. Le
Oksana V. Kozlova
Natalia S. Velichkovich
Anastasiya M. Fedorova
Anna I. Loseva
Vladimir P. Yustratov
spellingShingle Irina S. Milentyeva
Violeta M. Le
Oksana V. Kozlova
Natalia S. Velichkovich
Anastasiya M. Fedorova
Anna I. Loseva
Vladimir P. Yustratov
Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics
Foods and Raw Materials
callus culture
cell suspension culture
hairy roots
medicinal plant
secondary metabolite
phenolic substances
antioxidant
oxidative stress
antimicrobial properties
extraction
author_facet Irina S. Milentyeva
Violeta M. Le
Oksana V. Kozlova
Natalia S. Velichkovich
Anastasiya M. Fedorova
Anna I. Loseva
Vladimir P. Yustratov
author_sort Irina S. Milentyeva
title Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics
title_short Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics
title_full Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics
title_fullStr Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics
title_full_unstemmed Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics
title_sort secondary metabolites in in vitro cultures of siberian medicinal plants: content, antioxidant properties, and antimicrobial characteristics
publisher Kemerovo State University
series Foods and Raw Materials
issn 2308-4057
2310-9599
publishDate 2021-04-01
description Introduction. Wild-crafting leads to the local extinction of many medicinal plants that are rich in phenolic substances. In vitro cultivation of cells and organs of higher plants can be the optimal solution to this problem. The research objective was to study the biosynthetic activity of in vitro extracts of wild Siberian plants. Study objects and methods. The study featured callus, cell suspension, and hairy root extracts of such Siberian medicinal plants as Eleutherococcus senticosus, Codonopsis pilosula, Platanthera bifolia, and Saposhnikovia divaricata. They were obtained by in vitro cultivation using modified nutrient media of Murashige and Skoog and Gamborg. The content of secondary metabolites was studied using the methods of thin-layer and high-performance liquid chromatography. A set of in vitro experiments tested the antioxidant and antimicrobial activity of the extracts. Results and discussion. All the samples demonstrated a high content of secondary metabolites of phenolic nature. Flavonoglycosides, apigenin, and rutin were found to be the predominant biologically active substances in the callus extracts. Flavonoglycosides dominated in the suspension extracts. The root extracts contained more caffeic acid, rutin, ecdysteroids, quercetin, apigenin, cardiofolin, and coleofolide than the callus and suspension cultures. The list of prevailing secondary metabolites in the root extracts included rutin, apigenin, coleofolide, and quercetin. All the extracts showed antimicrobial and antioxidant activity. Conclusion. All the extracts demonstrated good antioxidant and antimicrobial properties. Therefore, they can be used for the production of pharmaceuticals and biologically active food supplements as they can be helpful against infectious diseases, as well as oncological, cardiovascular, and neurodegenerative diseases linked to oxidative stress.
topic callus culture
cell suspension culture
hairy roots
medicinal plant
secondary metabolite
phenolic substances
antioxidant
oxidative stress
antimicrobial properties
extraction
url http://jfrm.ru/files/archive/17/Milentyeva.pdf
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