A silicic acid adsorption method for the determination of ganglioside sialic acid*

A semimicro method for the analysis of ganglioside sialic acid is presented. The method is based upon the fact that brain gangliosides can be adsorbed on a small amount of silicic acid from 92% n-butanol and eluted with methanol. Recovery of added gangliosides was found to be 93.5%, and the coeffici...

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Main Authors:	R.H. Mccluer, E.H. Coram, H.S. Lee
Format:	Article
Language:	English
Published:	Elsevier 1962-04-01
Series:	Journal of Lipid Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520404407

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record_format	Article
spelling	doaj-14540e51f84145cfbffcdbd17333da6f2021-04-23T06:13:08ZengElsevierJournal of Lipid Research0022-22751962-04-0132269274A silicic acid adsorption method for the determination of ganglioside sialic acid*R.H. Mccluer0E.H. Coram1H.S. Lee2Columbus Psychiatric Institute and Hospital and Department of Physiological Chemistry, Ohio State University, Columbus 10, OhioColumbus Psychiatric Institute and Hospital and Department of Physiological Chemistry, Ohio State University, Columbus 10, OhioColumbus Psychiatric Institute and Hospital and Department of Physiological Chemistry, Ohio State University, Columbus 10, OhioA semimicro method for the analysis of ganglioside sialic acid is presented. The method is based upon the fact that brain gangliosides can be adsorbed on a small amount of silicic acid from 92% n-butanol and eluted with methanol. Recovery of added gangliosides was found to be 93.5%, and the coefficient of variation for the analysis of 5 to 50 mg of tissue was found to be ±4.4%.http://www.sciencedirect.com/science/article/pii/S0022227520404407
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	R.H. Mccluer E.H. Coram H.S. Lee
spellingShingle	R.H. Mccluer E.H. Coram H.S. Lee A silicic acid adsorption method for the determination of ganglioside sialic acid* Journal of Lipid Research
author_facet	R.H. Mccluer E.H. Coram H.S. Lee
author_sort	R.H. Mccluer
title	A silicic acid adsorption method for the determination of ganglioside sialic acid*
title_short	A silicic acid adsorption method for the determination of ganglioside sialic acid*
title_full	A silicic acid adsorption method for the determination of ganglioside sialic acid*
title_fullStr	A silicic acid adsorption method for the determination of ganglioside sialic acid*
title_full_unstemmed	A silicic acid adsorption method for the determination of ganglioside sialic acid*
title_sort	silicic acid adsorption method for the determination of ganglioside sialic acid*
publisher	Elsevier
series	Journal of Lipid Research
issn	0022-2275
publishDate	1962-04-01
description	A semimicro method for the analysis of ganglioside sialic acid is presented. The method is based upon the fact that brain gangliosides can be adsorbed on a small amount of silicic acid from 92% n-butanol and eluted with methanol. Recovery of added gangliosides was found to be 93.5%, and the coefficient of variation for the analysis of 5 to 50 mg of tissue was found to be ±4.4%.
url	http://www.sciencedirect.com/science/article/pii/S0022227520404407
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A silicic acid adsorption method for the determination of ganglioside sialic acid*

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