Functional characterization of the origin of replication of pRN1 from Sulfolobus islandicus REN1H1.

Plasmid pRN1 from Sulfolobus islandicus REN1H1 is believed to replicate by a rolling circle mechanism but its origin and mechanism of replication are not well understood. We sought to create minimal expression vectors based on pRN1 that would be useful for heterologous gene expression in S. acidocal...

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Bibliographic Details
Main Authors: Chijioke J Joshua, Luis D Perez, Jay D Keasling
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3869888?pdf=render

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